{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T19:28:40Z","timestamp":1789932520412,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,8,23]],"date-time":"2018-08-23T00:00:00Z","timestamp":1534982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Supherb Ltd, Israel"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although probiotics are increasingly used in irritable bowel syndrome (IBS), their mechanism of action has not been elucidated sufficiently. We aimed to evaluate the impact of a multispecies probiotic on enteric microbiota composition in women with diarrhea\u2010predominant\u2010IBS (IBS\u2010D) and to determine whether these effects are associated with changes in IBS symptoms or inflammatory markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In a double\u2010blind, placebo\u2010controlled study, Rome III IBS\u2010D women completed a two\u2010week run\u2010in period and eligible women were assigned at random to a probiotic capsule (BIO\u201025) or an indistinguishable placebo, twice daily for 8\u00a0weeks. IBS symptoms and stool consistency were rated daily by visual analogue scales and the Bristol stool scale. High sensitivity C\u2010reactive protein, fecal calprotectin and microbial composition were tested at baseline and at 4 and 8\u00a0weeks. Microbial sequencing of the 16S rRNA was performed and data were analyzed to compare patients who responded to treatment with those who did not.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>172 IBS\u2010D patients were recruited and 107 eligible patients were allocated to the intervention (n\u00a0=\u00a054) or placebo (n\u00a0=\u00a053) group. Compared to placebo, BIO\u201025 did not result in changes in microbial diversity or taxa proportions, except for higher relative proportions of <jats:italic>Lactobacillus<\/jats:italic> in the BIO\u201025 group (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.002). Symptomatic responders to BIO\u201025 showed a reduction in the proportion of <jats:italic>Bilophila<\/jats:italic>(<jats:italic>P<\/jats:italic>\u00a0=\u00a00.003) posttreatment. Patients with beneficial inflammatory\u2010marker changes had higher baseline proportions of <jats:italic>Faecalibacterium<\/jats:italic>(<jats:italic>P<\/jats:italic>\u00a0=\u00a00.03), <jats:italic>Leuconostoc<\/jats:italic> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.03), <jats:italic>and Odoribacter<\/jats:italic> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.05) compared to corresponding non\u2010responders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Identifying patients with a more amenable microbiome at treatment initiation may result in better treatment response.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13456","type":"journal-article","created":{"date-parts":[[2018,8,23]],"date-time":"2018-08-23T08:55:20Z","timestamp":1535014520000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["The effect of a multispecies probiotic on microbiota composition in a clinical trial of patients with diarrhea\u2010predominant irritable bowel syndrome"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8115-7274","authenticated-orcid":false,"given":"Keren","family":"Hod","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine Tel Aviv University Tel Aviv Israel"},{"name":"Research Division, Epidemiology Service Assuta Medical Centers Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy","family":"Dekel","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Liver Diseases, Tel\u2010Aviv Sourasky Medical Center, Affiliated to the Sackler Faculty of Medicine Tel Aviv University Tel Aviv Isreal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel","family":"Aviv Cohen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Liver Diseases, Tel\u2010Aviv Sourasky Medical Center, Affiliated to the Sackler Faculty of Medicine Tel Aviv University Tel Aviv Isreal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ami","family":"Sperber","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences Ben\u2010Gurion University of the Negev Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishai","family":"Ron","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Liver Diseases, Tel\u2010Aviv Sourasky Medical Center, Affiliated to the Sackler Faculty of Medicine Tel Aviv University Tel Aviv Isreal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Boaz","sequence":"additional","affiliation":[{"name":"Department of Nutrition Sciences, School of Health Sciences Ariel University Ariel Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shlomo","family":"Berliner","sequence":"additional","affiliation":[{"name":"Department of Medicine E, Tel Aviv Sourasky Medical Center, Affiliated to the Sackler Faculty of Medicine Tel Aviv University Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitsan","family":"Maharshak","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Liver Diseases, Tel\u2010Aviv Sourasky Medical Center, Affiliated to the Sackler Faculty of Medicine Tel Aviv University Tel Aviv Isreal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gtc.2016.09.014"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2011.01779.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312297"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2009.06.031"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2017.01.142"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.giec.2008.12.004"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301501"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.09.049"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.043"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2016.1218585"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15006-017-0338-3"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13037"},{"key":"e_1_2_10_14_1","unstructured":"CfDEaR (CDER).Guidance for Industry Irritable Bowel Syndrome \u2014 Clinical Evaluation of Products for Treatment. 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