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Control samples (n\u00a0=\u00a06 ileum and n\u00a0=\u00a06 jejunum) were used to establish the range of normality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Fourteen parameters were defined to assess muscle contractility and nerve\u2010muscle interaction: five to evaluate smooth muscle and interstitial cells of Cajal (ICC) and nine to evaluate inhibitory neuromuscular transmission. For each sample, a parameter was scored 0 if the value was inside the normal range or a value of 1 if it was outside. Patients\u2019 samples (CIPO\/ED) had more abnormal parameters than controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001 for both jejunum and ileum). Functional abnormalities were found to be heterogeneous. The most prevalent abnormality was a decreased purinergic neuromuscular transmission, which was detected in 43.8% of jejunal samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>Abnormalities of neuromuscular intestinal function are detected in vitro in severe intestinal dysmotility. However, consistent with the heterogeneity of the disease pathophysiology, functional impairment cannot be attributed to a single mechanism. Specifically, defects of purinergic neuromuscular transmission may have an important role in motility disorders of the gastrointestinal tract.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13458","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T06:03:44Z","timestamp":1536041024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Functional neuromuscular impairment in severe intestinal dysmotility"],"prefix":"10.1111","volume":"30","author":[{"given":"Diana","family":"Gallego","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Physiology and Immunology and Neurosciences Institute Universitat Aut\u00f2noma de Barcelona Barcelona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBEREHD) Barcelona 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