{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:36:29Z","timestamp":1790174189608,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"R\u00e9gion des pays de la Loire","award":["GEPHIN2"],"award-info":[{"award-number":["GEPHIN2"]}]},{"name":"Association Fran\u00e7ois Aupetit"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neuroimmune interactions are essential to maintain gut homeostasis and prevent intestinal disorders but so far, the impact of enteric glial cells (EGC) on immune cells remains a relatively unexplored area of research. As a dysregulation of critical cytokines such as interleukine\u20107 (IL\u20107) was suggested to exacerbate gut chronic inflammation, we investigated whether EGC could be a source of IL\u20107 in the gastrointestinal tract.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Expression of IL\u20107 in the rat enteric nervous system was analyzed by immunochemistry and Q\u2010PCR. IL\u20107 variants were cloned and specific antibodies against rat IL\u20107 isoforms were raised to characterize their expression in the submucosal plexus. IL\u20107 isoforms were produced in vitro to analyze their impact on T\u2010cell survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Neurons and glial cells of the rat enteric nervous system expressed IL\u20107 at both mRNA and protein levels. Novel rat IL\u20107 isoforms with distinct C\u2010terminal parts were detected. Three of these isoforms were found in EGC or in both enteric neurons and EGC. Exposure of EGC to pro\u2010inflammatory cytokines (IL\u20101\u03b2 and\/or TNF\u03b1) induced an upregulation of all IL\u20107 isoforms. Interestingly, time\u2010course and intensity of the upregulation varied according to the presence or absence of exon 5a in IL\u20107 variants. Functional analysis on T lymphocytes revealed that only canonical IL\u20107 protects T cells from cell death.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>IL\u20107 and its variants are expressed by neurons and glial cells in the enteric nervous system. Their distinct expression and upregulation in inflammatory conditions suggest a role in gut homeostasis which could be critical in case of chronic inflammatory diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13467","type":"journal-article","created":{"date-parts":[[2018,9,22]],"date-time":"2018-09-22T02:28:18Z","timestamp":1537583298000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Rat enteric glial cells express novel isoforms of Interleukine\u20107 regulated during inflammation"],"prefix":"10.1111","volume":"31","author":[{"given":"Laetitia","family":"Kermarrec","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Nantes, INSERM Institut des Maladies de l'Appareil Digestif, The enteric nervous system in gut and brain disorders  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Durand","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, INSERM Institut des Maladies de l'Appareil Digestif, The enteric nervous system in gut and brain disorders  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Gonzales","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, INSERM Institut des Maladies de l'Appareil Digestif, The enteric nervous system in gut and brain disorders  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Pabois","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, INSERM Institut des Maladies de l'Appareil Digestif, The enteric nervous system in gut and brain disorders  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Hulin","sequence":"additional","affiliation":[{"name":"Plateforme MicroPICell SFR Sant\u00e9 Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1892-5071","authenticated-orcid":false,"given":"Michel","family":"Neunlist","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, INSERM Institut des Maladies de l'Appareil Digestif, The enteric nervous system in gut and brain disorders  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Neveu","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, INSERM Institut des Maladies de l'Appareil Digestif, The enteric nervous system in gut and brain disorders  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4093-4513","authenticated-orcid":false,"given":"Philippe","family":"Naveilhan","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, INSERM Institut des Maladies de l'Appareil Digestif, The enteric nervous system in gut and brain disorders  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22795"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP271021"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.09.040"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.221"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81571-8"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.07.002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.219519"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2015.08.004"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00067.2006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.09.038"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.188409"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.219717"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2749"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.01.051"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18644"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90832-J"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22036"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.6.G1163"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e3182a86fd4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2013.08.047"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2013-305005"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2013.09.030"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.3.389"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90400-H"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/362062a0"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-05-01753.1998"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2009.77"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118002"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12200"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.149815"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12414"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1199"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/286736a0"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0255"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2007.11.003"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/107999099313947"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2010.02.014"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2010.03.003"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2002.1974"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2008.10.019"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.5.1873"},{"issue":"154","key":"e_1_2_8_44_1","first-page":"6031","article-title":"IFN\u2010gamma\u2010dependent IL\u20107 gene regulation in keratinocytes","volume":"1995","author":"Ariizumi K","year":"1950","journal-title":"J Immunol"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.14.6298-6310.2004"},{"issue":"178","key":"e_1_2_8_46_1","first-page":"262","article-title":"IL\u20107\/STAT5 cytokine signaling pathway is essential but insufficient for maintenance of naive CD4 T cell survival in peripheral lymphoid organs","volume":"2007","author":"Seki Y\u2010I","year":"1950","journal-title":"J Immunol"},{"issue":"163","key":"e_1_2_8_47_1","first-page":"1817","article-title":"Strong TCR ligation without costimulation causes rapid onset of Fas\u2010dependent apoptosis of naive murine CD4+ T cells","volume":"1999","author":"Kishimoto H","year":"1950","journal-title":"J Immunol"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2008.90"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/11.1.31"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.13467","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.13467","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nmo.13467","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.13467","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T02:05:19Z","timestamp":1694743519000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.13467"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,21]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/nmo.13467"],"URL":"https:\/\/doi.org\/10.1111\/nmo.13467","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,21]]},"assertion":[{"value":"2018-02-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13467"}}