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The mechanisms underlying the hypersensitivity are not clear. Gastric vagal afferents (GVAs) play an important role in sensing meal\u2010related mechanical stimulation to modulate gastrointestinal function and food intake. This study aimed to determine whether GVAs display hypersensitivity after chronic stress, and whether its interaction with leptin was altered by stress.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eight\u2010week\u2010old male C57BL\/6 mice were exposed to unpredictable chronic mild stress or no stress (control) for 8\u00a0weeks. The metabolic rate, gastric emptying rate, and anxiety\u2010 and depression\u2010like behaviors were determined. GVA mechanosensitivity, and its modulation by leptin, was determined using an in vitro single fiber recording technique. QRT\u2010PCR was used to establish the levels of leptin and leptin receptor mRNA in the stomach and nodose ganglion, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The stressed mice had lower body weight and food intake, and increased anxiety\u2010like behavior compared to the control mice. The mechanosensitivity of mucosal and tension\u2010sensitive GVAs was higher in the stressed mice. Leptin potentiated mucosal GVA mechanosensitivity in control but not stressed mice. The expression of leptin mRNA in the gastric mucosa was lower in the stressed mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>In conclusion, chronic stress enhances GVA mechanosensitivity, which may contribute to the gastric hypersensitivity in FD. In addition, the modulatory effect of leptin on GVA signaling is lost after chronic stress exposure.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13669","type":"journal-article","created":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T12:40:30Z","timestamp":1561552830000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Chronic stress induces hypersensitivity of murine gastric vagal afferents"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1010-516X","authenticated-orcid":false,"given":"Hui","family":"Li","sequence":"first","affiliation":[{"name":"Vagal Afferent Research Group, Centre for Nutrition and Gastrointestinal Disease, Adelaide Medical School University of Adelaide Adelaide South Australia Australia"},{"name":"Nutrition, Diabetes and Metabolism South Australian Health and Medical Research Institute Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Femke T. 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