{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:15:26Z","timestamp":1767374126764,"version":"3.41.2"},"reference-count":58,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T00:00:00Z","timestamp":1563235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001636","name":"University College Cork","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001636","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Patients with irritable bowel syndrome (IBS) may experience postprandial symptom exacerbation. Nutrients stimulate intestinal release of glucagon\u2010like peptide 1 (GLP\u20101), an incretin hormone with known gastrointestinal effects. However, prior to the postprandial rise in GLP\u20101, levels of the hunger hormone, ghrelin, peak. The aims of this study were to determine if ghrelin sensitizes colonic intrinsic and extrinsic neurons to the stimulatory actions of a GLP\u20101 receptor agonist, and if this differs in a rat model of IBS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Calcium imaging of enteric neurons was compared between Sprague Dawley and Wistar Kyoto rats. Colonic contractile activity and vagal nerve recordings were also compared between strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Circulating GLP\u20101 concentrations differ between IBS subtypes. Mechanistically, we have provided evidence that calcium responses evoked by exendin\u20104, a GLP\u20101 receptor agonist, are potentiated by a ghrelin receptor (GHSR\u20101) agonist, in both submucosal and myenteric neurons. Although basal patterns of colonic contractility varied between Sprague Dawley and Wister Kyoto rats, the capacity of exendin\u20104 to alter smooth muscle function was modified by a GHSR\u20101 agonist in both strains. Gut\u2010brain signaling via GLP\u20101\u2013mediated activation of vagal afferents was also potentiated by the GHSR\u20101 agonist.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>These findings support a temporal interaction between ghrelin and GLP\u20101, where the preprandial peak in ghrelin may temporarily sensitize colonic intrinsic and extrinsic neurons to the neurostimulatory actions of GLP\u20101. While the sensitizing effects of the GHSR\u20101 agonist were identified in both rat strains, in the rat model of IBS, underlying contractile activity was aberrant.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13684","type":"journal-article","created":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T23:53:49Z","timestamp":1563321229000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["GHSR\u20101 agonist sensitizes rat colonic intrinsic and extrinsic neurons to exendin\u20104: A role in the manifestation of postprandial gastrointestinal symptoms in irritable bowel syndrome?"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9097-1334","authenticated-orcid":false,"given":"Maria M.","family":"Buckley","sequence":"first","affiliation":[{"name":"Department of Physiology University College Cork Cork Ireland"},{"name":"APC Microbiome Ireland Biosciences Institute, University College 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