{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:10:59Z","timestamp":1767856259973,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T00:00:00Z","timestamp":1574726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Ministry of Health"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Achalasia is a rare idiopathic disease with a complex etio\u2010pathogenesis still unknown. This study aimed to identify microRNA (miRNA)\u2010mRNA regulatory networks underlying achalasia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The investigation was performed in tissue specimens from 11 patients and five controls using the microarray technology followed by an integrated bioinformatics analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>One hundred and six miRNAs were significantly up\u2010regulated and 64 were down\u2010regulated in achalasia patients. The expression of the most 10 differential expressed miRNAs (miR\u2010122\u20105p, miR\u2010133a\u20103p, miR\u2010504\u20105p, miR\u2010187\u20103p, miR\u2010133b, miR\u2010200c\u20103p, miR\u2010375, miR\u2010200b\u20105p, miR\u2010200b\u20103p, and miR203a) was confirmed by droplet digital PCR in an independent cohort. The interactions between the significant miRNAs and their targets uncovered 14 miRNA\u2010mRNA interacting pairs with experimentally predicted genes (ie, <jats:italic>FN1<\/jats:italic>, <jats:italic>ROCK2<\/jats:italic>, <jats:italic>DPYSL2<\/jats:italic>), and 35 pairs with not experimentally target genes (ie, <jats:italic>SULF1<\/jats:italic>, <jats:italic>MRVI1<\/jats:italic>, <jats:italic>PRKG1<\/jats:italic>); all genes were involved in <jats:italic>immune cell trafficking<\/jats:italic>, <jats:italic>skeletal and muscular system development<\/jats:italic>, <jats:italic>nervous system development<\/jats:italic> macro\u2010processes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion &amp; Inferences<\/jats:title><jats:p>The mRNA\u2013miRNA regulatory networks described in this study provide new insights in the genetic background of the disease, suggesting further investigations in novel pathogenic mechanisms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13764","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T02:09:31Z","timestamp":1574820571000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["microRNA\u2010mRNA network model in patients with achalasia"],"prefix":"10.1111","volume":"32","author":[{"given":"Orazio","family":"Palmieri","sequence":"first","affiliation":[{"name":"Gastroenterology Unit Fondazione IRCCS Casa Sollievo della Sofferenza San Giovanni Rotondo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tommaso","family":"Mazza","sequence":"additional","affiliation":[{"name":"Bioinformatics Unit Fondazione IRCCS Casa Sollievo della Sofferenza San Giovanni Rotondo 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