{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T02:04:56Z","timestamp":1778724296495,"version":"3.51.4"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T00:00:00Z","timestamp":1582502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>BALB\/c and C57BL\/6\u00a0mice are widely used in biomedical research; however, the differences between strains are still underestimated.\u00a0Our aims were to develop an experimental protocol to evaluate the duodenal contractility and gastrointestinal transit in mice\u00a0using the\u00a0Alternating Current Biosusceptometry (ACB)\u00a0technique\u00a0and to compare gastrointestinal motor function and morphology between BALB\/c and C57BL\/6 strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male mice were\u00a0used in\u00a0experiments (a) duodenal contractility: animals\u00a0which had a magnetic marker surgically fixed in the duodenum to determine the frequency and amplitude of contractions and (b) gastrointestinal transit: animals which ingested a magnetically marked chow\u00a0to calculate the\u00a0Oro\u2010Anal Transit Time (OATT) and the Fecal Pellet Elimination Rate (FPER). The animals were killed\u00a0after the experiments for organ collection and\u00a0morphometric analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>BALB\/c and C57BL\/6 had two different duodenal frequencies (high and low)\u00a0with similar amplitudes. After 10\u00a0hours of monitoring, BALB\/c eliminated around 89% of the ingested\u00a0marker and C57BL\/6 eliminated\u00a033%; OATT and FPER were slower for C57BL\/6 compared with BALB\/c. The OATT and amplitude of low frequency\u00a0had a strong positive correlation in C57BL\/6. For BALB\/c, the\u00a0gastric muscular layer was thicker\u00a0compared to that measured for C57BL\/6.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>The experimental protocol\u00a0to evaluate duodenal contractility and fecal magnetic pellets output using the ACB technique\u00a0in mice\u00a0was successfully established.\u00a0BALB\/c strains had higher duodenal frequencies and a shorter time\u00a0to eliminate the ingested marker. Our results\u00a0showed differences in both\u00a0motor function and gastrointestinal morphology between\u00a0BALB\/c and C57BL\/6 strains.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13824","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T07:07:12Z","timestamp":1582614432000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Gastrointestinal motility and morphology in mice: Strain\u2010dependent differences"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8118-1047","authenticated-orcid":false,"given":"Loyane Almeida","family":"Gama","sequence":"first","affiliation":[{"name":"Institute of Biosciences S\u00e3o Paulo State University UNESP Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8412-2483","authenticated-orcid":false,"given":"Mariana Pirani","family":"Rocha 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