{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:57:43Z","timestamp":1789189063302,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T00:00:00Z","timestamp":1588809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1088737"],"award-info":[{"award-number":["1088737"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Enterochromaffin (EC) cells are specialized enteroendocrine cells lining the gastrointestinal (GI) tract and the source of almost all serotonin (5\u2010hydroxytryptamine; 5\u2010HT) in the body. Gut\u2010derived 5\u2010HT has a plethora of physiological roles, including regulation of gastrointestinal motility, and has been implicated as a driver of obesity and metabolic disease. This is due to 5\u2010HT influencing key metabolic processes, such as hepatic gluconeogenesis, adipose tissue lipolysis and hindering thermogenic capacity. Increased circulating 5\u2010HT occurs in humans with obesity and type 2 diabetes. However, despite the known metabolic roles of gut\u2010derived 5\u2010HT, the mechanisms underlying the cellular\u2010level change in EC cells under obesogenic conditions remains unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We use a mouse model of diet\u2010induced obesity (DIO) to identify the regional changes that occur in primary EC cells from the duodenum and colon. Transcriptional changes in the nutrient sensing profile of primary EC cells were assessed, and responses to nutrient stimuli in culture were determined by 5\u2010HT ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>We find that obesogenic conditions affect EC cells in a region\u2010dependent manner. Duodenal EC cells from DIO mice have impaired sugar sensing even in the presence of increased 5\u2010HT content per cell, while colonic EC cell numbers are significantly increased, but have unaltered nutrient sensing capacity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Our findings from this study add novel insights into the mechanisms by which functional changes to EC cells occur at a cellular level, which may contribute to the altered circulating 5\u2010HT seen with obesity and metabolic disease, and associated gastrointestinal disorders.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13869","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T11:32:00Z","timestamp":1588851120000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Diet differentially regulates enterochromaffin cell serotonin content, density and nutrient sensitivity in 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