{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T09:28:36Z","timestamp":1791365316882,"version":"4.3.1"},"reference-count":47,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T00:00:00Z","timestamp":1588723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1156416"],"award-info":[{"award-number":["1156416"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Colonic high\u2010resolution manometry (HRM) has been used to reveal discrete, propagating colonic motor patterns. To help determine mechanisms underlying these patterns, we used HRM to record contractile activity in human distal colon ex vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Surgically excised segments of descending (n\u00a0=\u00a030) or sigmoid colon (n\u00a0=\u00a04) were immersed in oxygenated Krebs solution at 36\u00b0C (n\u00a0=\u00a034; 16 female; 67.6\u00a0\u00b1\u00a012.4\u00a0years; length: 24.7\u00a0\u00b1\u00a03.5\u00a0cm). Contractility was recorded by HRM catheters. After 30\u00a0minutes of baseline recording, 0.3\u00a0mM lidocaine and\/or 1\u00a0mM hexamethonium were applied. Ascending neural pathways were activated by electrical field stimulation (EFS; 10\u00a0Hz, 0.5\u00a0ms, 50\u00a0V, 5\u2010s duration) applied to the anal end before and after drug application.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Spontaneous propagating contractions were recorded in all specimens (0.1\u20101.5 cycles\/minute). Most contractions occurred synchronously across all recording sites. In five specimens, rhythmic antegrade contractions propagated across\u00a0the full length of the preparation. EFS evoked local contractions at the site of stimulation (latency: 5.5\u00a0\u00b1\u00a02.4\u00a0seconds) with greater amplitude than spontaneous contractions (EFS; 29.3\u00a0\u00b1\u00a026.9 vs 12.1\u00a0\u00b1\u00a014.8\u00a0mm Hg; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.02).\u00a0Synchronous or retrograde propagating motor patterns followed EFS; 71% spanned the entire preparation length. Hexamethonium and lidocaine modestly and only temporarily inhibited spontaneous contractions, whereas TTX increased the frequency of contractile activity while inhibiting EFS\u2010evoked contractions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>Our study suggests that the propagated contractions recorded in the organ bath have a myogenic origin which can be regulated by neural input. Once activated at a local site, the contractions do not require the propulsion of fecal content to sustain long\u2010distance propagation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13871","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T10:24:06Z","timestamp":1588760646000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Distinct patterns of myogenic motor activity identified in isolated human distal colon with high\u2010resolution manometry"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8727-7747","authenticated-orcid":false,"given":"Reizal M.","family":"Rosli","sequence":"first","affiliation":[{"name":"College of Medicine and Public Health &amp; Centre for Neuroscience Flinders University Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2991-2762","authenticated-orcid":false,"given":"Paul T.","family":"Heitmann","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health &amp; Centre for Neuroscience Flinders University Adelaide SA Australia"},{"name":"Departments of Surgery and Gastroenterology Flinders Medical Centre Bedford Park SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghu","family":"Kumar","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Gastroenterology Flinders Medical Centre Bedford Park SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim J.","family":"Hibberd","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health &amp; Centre for Neuroscience Flinders University Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Costa","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health &amp; Centre for Neuroscience Flinders University Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukasz","family":"Wiklendt","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health &amp; Centre for Neuroscience Flinders University Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Wattchow","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health &amp; Centre for Neuroscience Flinders University Adelaide SA Australia"},{"name":"Departments of Surgery and Gastroenterology Flinders Medical Centre Bedford Park SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6460-6499","authenticated-orcid":false,"given":"John","family":"Arkwright","sequence":"additional","affiliation":[{"name":"College of Science and Engineering Flinders University Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayan","family":"de Fontgalland","sequence":"additional","affiliation":[{"name":"Departments of Surgery and Gastroenterology Flinders Medical Centre Bedford Park SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5635-0876","authenticated-orcid":false,"given":"Simon J. 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