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Ingested nutrients are detected by specific gut chemosensors. However, knowledge of these chemosensors has primarily been derived from the intestine, while available information on gastric chemosensors is limited. This study aimed to investigate the nutrient\u2010sensing repertoire of the mouse stomach with particular emphasis on ghrelin cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative RT\u2010PCR was used to determine mRNA levels of nutrient chemosensors (protein: G protein\u2013coupled receptor 93 [GPR93], calcium\u2010sensing receptor [CaSR], metabotropic glutamate receptor type 4 [mGluR4]; fatty acids: CD36, FFAR2&amp;4; sweet\/umami taste: T1R3), taste transduction components (TRPM5, GNAT2&amp;3), and ghrelin and ghrelin\u2010processing enzymes (PC1\/3, ghrelin O\u2010acyltransferase [GOAT]) in the gastric corpus and antrum of adult male C57BL\/6 mice. Immunohistochemistry was performed to assess protein expression of chemosensors (GPR93, T1R3, CD36, and FFAR4) and their co\u2010localization with ghrelin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Most nutrient chemosensors had higher mRNA levels in the antrum compared to the corpus, except for CD36, GNAT2, ghrelin, and GOAT. Similar regional distribution was observed at the protein level. At least 60% of ghrelin\u2010positive cells expressed T1R3 and FFAR4, and over 80% expressed GPR93 and CD36.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>The cellular mechanisms for the detection of nutrients are expressed in a region\u2010specific manner in the mouse stomach and gastric ghrelin cells. These gastric nutrient chemosensors may play a role modulating gastrointestinal responses, such as the inhibition of ghrelin secretion following food intake.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13944","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T03:23:46Z","timestamp":1594783426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Nutrient\u2010sensing components of the mouse stomach and the gastric ghrelin cell"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1879-7797","authenticated-orcid":false,"given":"Maria","family":"Nunez\u2010Salces","sequence":"first","affiliation":[{"name":"Vagal Afferent Research Group Adelaide Medical School The University of Adelaide  Adelaide SA Australia"},{"name":"Centre of Research Excellence in Translating Nutritional Science to Good Health Adelaide Medical School The University of Adelaide  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