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Slow colonic transit and constipation are common in DMD patients and animal models of DMD. However, the cause of this hypocontractility and the expression of contractile proteins in smooth muscle are unknown. The aim of the study was to investigate the expression of contractile proteins in the colonic smooth muscle and the function of the colon in control and <jats:italic>mdx<\/jats:italic> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Muscle contraction was measured in muscle strips and isolated muscle cells. Peristaltic activity was measured in ex vivo preparations by spatiotemporal mapping, and gastrointestinal (GI) transit in vivo was measured by the distribution of fluorescent marker along the intestine and colon. mRNA expression of contractile proteins smoothelin, caldesmon, calponin, and tropomyosin was measured by qRT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of mRNA for contractile proteins was decreased in colonic smooth muscle of <jats:italic>mdx mice<\/jats:italic> compared with control. Contraction in response to acetylcholine and KCl was decreased in colonic muscle strips and in isolated muscle cells of <jats:italic>mdx<\/jats:italic> mice. Distension of ex vivo colons with Krebs buffer induced peristalsis in both control and <jats:italic>mdx<\/jats:italic> mice; however, significantly fewer full peristaltic waves were recorded in the colons of <jats:italic>mdx<\/jats:italic> mice. GI transit was also inhibited in <jats:italic>mdx<\/jats:italic> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Inferences<\/jats:title><jats:p>The data indicate that the lack of dystrophin causes decrease in colonic smooth muscle contractility, peristalsis, and GI transit and provides the basis for analysis of mechanisms involved in smooth muscle dysfunction in DMD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13968","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T06:52:39Z","timestamp":1597301559000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Decreased smooth muscle function, peristaltic activity, and gastrointestinal transit in dystrophic (<i>mdx<\/i>) mice"],"prefix":"10.1111","volume":"33","author":[{"given":"Kulpreet","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics Virginia Commonwealth University  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