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Peripherally derived corticotropin\u2010releasing factor (CRF) is known to mediate stress\u2010induced intestinal secretion, presumably by activation of CRF\n                      <jats:sub>1<\/jats:sub>\n                      receptors in the gut. The present study aimed to ascertain the role of CRF\n                      <jats:sub>2<\/jats:sub>\n                      activation in intestinal secretion by three other members of CRF peptide family, urocortin (UCN) 1\u20133, in wild type (WT) and CRF\n                      <jats:sub>2<\/jats:sub>\n                      knockout (\n                      <jats:italic>Crhr2<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      ) mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Mucosal\/submucosal preparations from proximal colon of WT and\n                      <jats:italic>Crhr2<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice of both sexes were mounted in Ussing chambers for measurement of short\u2010circuit current (\n                      <jats:italic>\n                        I\n                        <jats:sub>sc<\/jats:sub>\n                      <\/jats:italic>\n                      ) as an indicator of ion secretion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      Male mice demonstrated a significantly higher baseline\n                      <jats:italic>\n                        I\n                        <jats:sub>sc<\/jats:sub>\n                      <\/jats:italic>\n                      than female in both WT and\n                      <jats:italic>Crhr2<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      genotypes. CRF and UCN1\u20103 (1\u00a0\u03bcM) caused greater increases in colonic\n                      <jats:italic>\n                        I\n                        <jats:sub>sc<\/jats:sub>\n                      <\/jats:italic>\n                      (\u0394\n                      <jats:italic>\n                        I\n                        <jats:sub>sc<\/jats:sub>\n                      <\/jats:italic>\n                      ) in male than female. Colonic\n                      <jats:italic>\n                        I\n                        <jats:sub>sc<\/jats:sub>\n                      <\/jats:italic>\n                      response to the selective CRF\n                      <jats:sub>1<\/jats:sub>\n                      agonist, stressin1, was similar in both sexes. In male mice, the selective CRF\n                      <jats:sub>2<\/jats:sub>\n                      agonists (UCN2 and UCN3) caused significantly greater \u0394\n                      <jats:italic>\n                        I\n                        <jats:sub>sc<\/jats:sub>\n                      <\/jats:italic>\n                      than CRF and stressin1. UCN2\u2010 and UCN3\u2010evoked \u0394\n                      <jats:italic>I<\/jats:italic>\n                      <jats:sub>SC<\/jats:sub>\n                      was significantly reduced in preparations pretreated with the selective CRF\n                      <jats:sub>2<\/jats:sub>\n                      antagonist antisauvagine\u201030 and in\n                      <jats:italic>Crhr2<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice. The prosecretory effects of urocortins were due to increases in Cl\n                      <jats:sup>\u2212<\/jats:sup>\n                      secretion and involved enteric neurons and mast cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Inference<\/jats:title>\n                    <jats:p>\n                      The findings revealed sex differences in baseline colonic secretion and responses to stress\u2010related peptides. CRF\n                      <jats:sub>2<\/jats:sub>\n                      receptors play a more prominent role in colonic secretion in male mice. The greater baseline secretion and responses to UCNs may contribute to the higher prevalence of diarrhea\u2010predominant IBS in males.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.14021","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T23:33:38Z","timestamp":1603928018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of stress\u2010related peptides on chloride secretion in the mouse proximal colon"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8769-429X","authenticated-orcid":false,"given":"Sumei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Biology College of Science and Health University of Wisconsin\u2010La Crosse  La Crosse WI 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