{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:28:11Z","timestamp":1785338891983,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570488 81770536"],"award-info":[{"award-number":["81570488 81770536"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Electroacupuncture (EA) at ST\u201036 could accelerate the delayed gastrointestinal (GI) motility in many GI motility dysfunction models, but the definite effect and mechanisms are unclear. In this study, we intended to investigate the effects of EA on intestinal manipulation (IM) mice model and involved mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male C57BL\/6 mice were randomized into five groups: normal control, intestinal manipulation (IM), IM with sham EA (SEA), IM with high\u2010frequency EA (HEA), and IM with low\u2010frequency EA (LEA). The GI transit was evaluated. The infiltration of muscularis macrophages (MM\u03c6) and its phenotype were analyzed with flow cytometry. Magnetic\u2010activated cell sorting was applied to isolate MM\u03c6, and the relationship between the MM\u03c6 and interstitial cells of Cajal (ICCs) was further investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>(1) Compared with the IM group, HEA and LEA attenuated the delayed intestinal transit. (2) Both the HEA and LEA obviously reduced the MM\u03c6 and suppressed the M1 activation of the MM\u03c6 in the ileum. (3) EA restored the disrupted ICC networks through inhibiting the release of IL6 by the MM\u03c6.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>(1) Electroacupuncture at acupoint ST\u201036 could accelerate the delayed intestinal transit in the IM murine model by restoring the ICC networks. (2) EA protected the ICCs through reducing the MM\u03c6, inhibiting its M1 polarization and its IL6 secretion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14066","type":"journal-article","created":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T06:37:09Z","timestamp":1611383829000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Electroacupuncture accelerates the delayed intestinal transit in POI by suppressing M1 like muscularis macrophages and IL6 secretion"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7402-8936","authenticated-orcid":false,"given":"Shuangning","family":"Song","sequence":"first","affiliation":[{"name":"Division of Gastroenterology Wuhan Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 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