{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:31:07Z","timestamp":1792452667022,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,1,26]],"date-time":"2021-01-26T00:00:00Z","timestamp":1611619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV\u201018\u20100283"],"award-info":[{"award-number":["APVV\u201018\u20100283"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003194","name":"Agent\u00fara Ministerstva \u0160kolstva, Vedy, V\u00fdskumu a \u0160portu SR","doi-asserted-by":"publisher","award":["VEGA 2\/0042\/19"],"award-info":[{"award-number":["VEGA 2\/0042\/19"]}],"id":[{"id":"10.13039\/501100003194","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Simultaneous evaluation of barrier protein expression in the gut and the brain and their modulation under stress conditions have not been studied before now. As the permeability and function of the gut and blood\u2010brain barrier are different and both express the MRs, we hypothesized that stress of post\u2010weaning social isolation induces changes in tight junction protein expression in the gut which are (1) independent of changes in the brain and (2) are mediated via the mineralocorticoid receptor (MR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>First, using UPLC\u2010MS\/MS we have successfully validated and selected a dose (1.2\u00a0mg\/rat\/day) of the MR antagonist spironolactone to treat female rats exposed to stress of chronic isolation or control conditions from postnatal day 21 for 9\u00a0weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>Isolation stress caused an enhancement of gene expression of occludin and ZO\u20101 and a decrease in claudin\u20105 and MR expression in both the small intestine and prefrontal cortex. Isolation stress failed to decrease claudin\u20105 (small intestine) and MR (prefrontal cortex) gene expression in spironolactone\u2010treated rats. MR blockade resulted in a decrease in claudin\u201015 expression in the small intestine. Anxiogenic effect of chronic stress, measured in elevated plus\u2010maze test, was partly prevented by spironolactone treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions &amp; Inferences<\/jats:title>\n                    <jats:p>Claudins, the main regulators of intestinal barrier permeability responded to chronic stress of social isolation and\/or simultaneous blockade of MR in female rats by alterations independent of changes in the brain cortex. The results suggest a physiological role of MR in the control of claudin expression in the small intestine, but not in the brain cortex.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.14084","type":"journal-article","created":{"date-parts":[[2021,1,26]],"date-time":"2021-01-26T15:21:01Z","timestamp":1611674461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Tight junction proteins in the small intestine and prefrontal cortex of female rats exposed to stress of chronic isolation starting early in life"],"prefix":"10.1111","volume":"33","author":[{"given":"Peter","family":"Karailiev","sequence":"first","affiliation":[{"name":"Institute of Experimental Endocrinology Biomedical Research Center of the Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasa","family":"Hlavacova","sequence":"additional","affiliation":[{"name":"Institute of Experimental Endocrinology Biomedical Research Center of the Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Chmelova","sequence":"additional","affiliation":[{"name":"Institute of Experimental Endocrinology Biomedical Research Center of the Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie Z. 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