{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:25:03Z","timestamp":1767374703062,"version":"3.41.2"},"reference-count":61,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The pro\u2010inflammatory cytokine, interleukin (IL)\u20106 is elevated in individuals with the functional bowel disorder, irritable bowel syndrome (IBS). IL\u20106 can independently modify intestinal secreto\u2010motor function, thereby contributing to IBS pathophysiology. Additionally, hormonal changes may underlie symptom flares. Post\u2010prandial exacerbation of IBS symptoms has been linked to secretion of the incretin hormone, glucagon\u2010like peptide\u20101 (GLP\u20101), which can also influence colonic secreto\u2010motor activity. This study aimed to ascertain if the effects of GLP\u20101 on colonic secretory and contractile activity was impacted by elevated IL\u20106 levels and if sensory signals regarding such changes were reflected in altered vagal afferent activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Colonic secretory currents and circular muscle contractile activity was investigated in Sprague Dawley rats using Ussing chamber and organ bath electrophysiology. Regional afferent signaling was assessed using extracellular electrophysiological recordings from colonic vagal afferents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Application of the GLP\u20101 receptor agonist, exendin\u20104 (Ex\u20104) in the presence of IL\u20106 potentiated colonic secretory currents and transepithelial resistance. Vagal afferent fibers originating in the submucosal layer exhibited larger responses to Ex\u20104 when IL\u20106 was also present. In contrast, co\u2010application of Ex\u20104 and IL\u20106 to gut\u2010bath chambers suppressed circular muscle contractile activity. The activity in extrinsic afferents originating in the colonic myenteric layer was similarly suppressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Application of Ex\u20104 in the presence of IL\u20106 had divergent modulatory effects on colonic secretion and contractile activity. Similar patterns were observed in vagal afferent signaling originating in the submucosal and myenteric neuronal layers, indicating regional afferent activity reflected immune\u2010 and endocrine\u2010mediated changes in colonic function.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14160","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T13:29:04Z","timestamp":1620134944000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Divergent effects of exendin\u20104 and interleukin\u20106 on rat colonic secretory and contractile activity are associated with changes in regional vagal afferent signaling"],"prefix":"10.1111","volume":"33","author":[{"given":"Rebecca","family":"O'Brien","sequence":"first","affiliation":[{"name":"Department of Physiology University College Cork Cork 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