{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:42:15Z","timestamp":1753879335123,"version":"3.41.2"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T00:00:00Z","timestamp":1634515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study investigated the antidiarrheal potential of the aqueous extract (AECR) and hydroalcoholic extract of\u00a0Campomanesia reitziana\u00a0leaves (HECR), its ethyl acetate (EAF) and dichloromethane fractions (DCMF), and myricitrin isolated from EAF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The total phenols and flavonoids were measured, followed by chromatography and myricitrin isolation. The 2,2\u2010diphenyl\u20101\u2010picryl\u2010hydrazyl scavenger activity, the cytotoxicity, and the effects on LPS\u2010induced nitrite production in intestinal epithelial cells (IEC\u20106) were quantified. The effect of HECR, EAF, DCMF, and AECR on intestinal motility (IT), gastric emptying (GE), and castor oil\u2010induced diarrhea in mice was determined, as well as its antimicrobial activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The administration of AECR 10% (10\u00a0ml\/kg, p.o), but not HECR (300\u00a0mg\/kg), reduced the GE and IT by 52 and 51%. The EAF and DCMF at 300\u00a0mg\/kg also reduced IT but did not change GE. Moreover, AECR and EAF, but not DCMF, inhibited the castor oil\u2010induced diarrhea and naloxone or metoclopramide pretreatment did not change these effects. Myricitrin did not change IT and the evacuation index of mice. Finally, the dry residue of AECR inhibited bacterial growth and EAF showed bacteriostatic activity against\u00a0<jats:italic>S.\u00a0aureus<\/jats:italic>,\u00a0<jats:italic>E.\u00a0coli<\/jats:italic>,\u00a0and\u00a0<jats:italic>S.\u00a0typhimurium<\/jats:italic>\u00a0and antifungal for\u00a0<jats:italic>C.\u00a0albicans<\/jats:italic>. However, none of the preparations alter the viability of\u00a0<jats:italic>Giardia<\/jats:italic> spp. trophozoites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The AECR and EAF can be effective to treat diarrhea acting through opioid\u2010 or dopaminergic type 2 receptor\u2010independent mechanisms and by its antimicrobial actions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14277","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T08:33:06Z","timestamp":1634805186000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Preparations from <i>Campomanesia reitziana<\/i> reduce the gastrointestinal motility and castor oil\u2010induced diarrhea in a non\u2010opioid and non\u2010dopaminergic pathway in mice and display antimicrobial activity in 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