{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:16:42Z","timestamp":1787015802818,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T00:00:00Z","timestamp":1679356800000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP190103525"],"award-info":[{"award-number":["DP190103525"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Specialized enterochromaffin (EC) cells within the mucosal lining of the gut synthesize and secrete almost all serotonin (5\u2010hydroxytryptamine, 5\u2010HT) in the body. Significantly lower amounts of 5\u2010HT are made by other peripheral tissues and serotonergic neurons within the enteric nervous system (ENS). EC cells are in close proximity to 5\u2010HT receptors in the ENS, and the role of 5\u2010HT as a modulator of gut motility, particularly colonic motor complexes, has been well defined. However, the relative contribution of neuronal 5\u2010HT to this process under resting and stimulus\u2010evoked conditions is unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, we combined the use of the selective serotonin transporter (SERT) inhibitor, fluoxetine, with two models of mucosal 5\u2010HT depletion\u2014surgical removal of the mucosa and our Tph1\n                      <jats:sup>Cre\/ERT2<\/jats:sup>\n                      ; Rosa26\n                      <jats:sup>DTA<\/jats:sup>\n                      mouse line\u2014to determine the relative contribution of neuronal and mucosal 5\u2010HT to resting and distension\u2010evoked colonic motility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Fluoxetine significantly reduced the frequency of colonic migrating complexes (CMCs) in flat\u2010sheet preparations with the mucosa present and in intact control Tph1\u2010DTA colons in which EC cells were present. No such effect was observed in mucosa\u2010free preparations or in intact Tph1\u2010DTA preparations lacking EC cell 5\u2010HT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and inferences<\/jats:title>\n                    <jats:p>We demonstrate that mucosal 5\u2010HT release plays an important role in distension\u2010evoked colonic motility, and that SERT inhibition no longer alters gut motility when EC cells are absent, thus demonstrating that ENS 5\u2010HT does not play a role in regulating gut motility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.14361","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T14:09:01Z","timestamp":1647871741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Distinguishing the contributions of neuronal and mucosal serotonin in the regulation of colonic motility"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1631-8307","authenticated-orcid":false,"given":"Alyce M.","family":"Martin","sequence":"first","affiliation":[{"name":"Flinders Health and Medical Research Institute College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren A.","family":"Jones","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology University of Nevada School of Medicine  Reno Nevada USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2190-5303","authenticated-orcid":false,"given":"Nick J.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute College of Medicine and Public Health Flinders University  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4196-1583","authenticated-orcid":false,"given":"Kenton M.","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology University of Nevada School of Medicine  Reno Nevada USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0861-8334","authenticated-orcid":false,"given":"Seungil","family":"Ro","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology University of Nevada School of Medicine  Reno Nevada USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0154-8305","authenticated-orcid":false,"given":"Damien J.","family":"Keating","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute College of Medicine and Public Health Flinders University  Adelaide South Australia 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