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We aimed at evaluating the catalytic activity of ACE and ACE2 in the intestinal content and corresponding intestinal tissue along the gut of Wistar Han rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Portions of the ileum, cecum, proximal colon, and distal colon, and the corresponding intestinal content were collected from Wistar Han rats. Enzyme activity was evaluated by fluorometric assays using different substrates: Hippuryl\u2010His\u2010Leu for ACE\u2010C\u2010domain, Z\u2010Phe\u2010His\u2010Leu for ACE\u2010N\u2010domain, and Mca\u2010APK(Dnp) for ACE2. ACE and ACE2 concentration was assessed by ELISA. Ratios concerning concentrations and activities were calculated to evaluate the balance of the RAAS. Statistical analysis was performed using Friedman test followed by Dunn's multiple comparisons test or Wilcoxon matched\u2010pairs test whenever needed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>ACE and ACE2 are catalytically active in the intestinal content along the rat gut. The ACE N\u2010domain shows higher activity than the C\u2010domain both in the intestinal content and in the intestinal tissue. ACE and ACE2 are globally more active in the intestinal content than in the corresponding intestinal tissue. There was a distal\u2010to\u2010proximal prevalence of ACE2 over ACE in the intestinal tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>This work is the first to report the presence of catalytically active ACE and ACE2 in the rat intestinal content, supporting future research on the regulatory role of the intestinal RAAS on gut function and a putative link to the microbiome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14598","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T13:23:29Z","timestamp":1681392209000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>ACE<\/scp> and <scp>ACE2<\/scp> catalytic activity in the fecal content along the gut"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7875-8351","authenticated-orcid":false,"given":"Mariana","family":"Ferreira\u2010Duarte","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology, Department of Drug Sciences Faculty of Pharmacy of the University of Porto (FFUP)  Porto Portugal"},{"name":"LAQV@REQUIMTE University of Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5117-6979","authenticated-orcid":false,"given":"Lilian Caroline Gon\u00e7alves","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Medicine, Discipline Nephrology Universidade Federal de S\u00e3o Paulo (UNIFESP\/EPM)  S\u00e3o Paulo 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