{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T20:42:43Z","timestamp":1757623363659,"version":"3.44.0"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,30]],"date-time":"2024-06-30T00:00:00Z","timestamp":1719705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Serving as a reservoir, the gastric fundus can expand significantly, with an initial receptive and a following adaptive relaxation, controlled by extrinsic and intrinsic reflex circuits, respectively. We hypothesize that mechanosensitive enteric neurons (MEN) are involved in the adaptive relaxation, which is initiated when a particular gastric volume and a certain stretch of the stomach wall is reached. To investigate whether the responsiveness of MEN in the gastric fundus is dependent on tissue stretch, we performed mechanical stimulations in stretched versus ganglia \u201cat rest\u201d.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Responses of myenteric neurons in the guinea pig gastric fundus were recorded with membrane potential imaging using Di\u20108\u2010ANEPPS. MEN were identified by small\u2010volume intraganglionic injection in ganglia stretched to different degrees using a self\u2010constructed stretching tool. Immunohistochemical staining identified the neurochemical phenotype of MEN. Hexamethonium and capsaicin were added to test their effect on recruited MEN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>In stretched compared to \u201cat rest\u201d ganglia, significantly more MEN were activated. The change in the ganglionic area correlated significantly with the number of additional recruited MEN. The additional recruitment of MEN was independent from nicotinic transmission and the ratio of active MEN in stretched ganglia shifted towards a nitrergic phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Inferences<\/jats:title><jats:p>The higher number of active MEN with increasing stretch of the ganglia and their greater share of nitrergic phenotype might indicate their contribution to the adaptive relaxation. Further experiments are necessary to address the receptors involved in mechanotransduction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14858","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T01:54:00Z","timestamp":1719798840000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The recruitment of mechanosensitive enteric neurons in the guinea pig gastric fundus is dependent on ganglionic stretch level"],"prefix":"10.1111","volume":"36","author":[{"given":"Sophia","family":"Mayr","sequence":"first","affiliation":[{"name":"Institute for Physiology and Cell Biology University of Veterinary Medicine Hannover, Foundation  Hannover Germany"},{"name":"Center for Systems Neuroscience (ZSN)  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin","family":"Elfers","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology University of Veterinary Medicine Hannover, Foundation  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7803-3556","authenticated-orcid":false,"given":"Gemma","family":"Mazzuoli\u2010Weber","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology University of Veterinary Medicine Hannover, Foundation  Hannover Germany"},{"name":"Center for Systems Neuroscience (ZSN)  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,30]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_12_2_1","DOI":"10.1152\/ajpgi.1984.247.3.G265"},{"doi-asserted-by":"publisher","key":"e_1_2_12_3_1","DOI":"10.1016\/s0301-0082(97)00051-8"},{"doi-asserted-by":"publisher","key":"e_1_2_12_4_1","DOI":"10.1113\/jphysiol.1989.sp017815"},{"doi-asserted-by":"publisher","key":"e_1_2_12_5_1","DOI":"10.1002\/cne.903530202"},{"doi-asserted-by":"publisher","key":"e_1_2_12_6_1","DOI":"10.1152\/ajplegacy.1911.29.2.267"},{"doi-asserted-by":"publisher","key":"e_1_2_12_7_1","DOI":"10.1073\/pnas.88.24.11490"},{"doi-asserted-by":"publisher","key":"e_1_2_12_8_1","DOI":"10.1113\/jphysiol.1898.sp000737"},{"doi-asserted-by":"publisher","key":"e_1_2_12_9_1","DOI":"10.1111\/j.1469-7793.1997.479be.x"},{"doi-asserted-by":"publisher","key":"e_1_2_12_10_1","DOI":"10.1540\/jsmr.33.81"},{"doi-asserted-by":"publisher","key":"e_1_2_12_11_1","DOI":"10.1038\/351477a0"},{"doi-asserted-by":"publisher","key":"e_1_2_12_12_1","DOI":"10.1111\/j.1469-7793.1997.197bo.x"},{"doi-asserted-by":"publisher","key":"e_1_2_12_13_1","DOI":"10.1113\/jphysiol.1963.sp007040"},{"doi-asserted-by":"publisher","key":"e_1_2_12_14_1","DOI":"10.1007\/bf01299923"},{"doi-asserted-by":"publisher","key":"e_1_2_12_15_1","DOI":"10.1159\/000201449"},{"doi-asserted-by":"publisher","key":"e_1_2_12_16_1","DOI":"10.1136\/gut.51.2.219"},{"doi-asserted-by":"publisher","key":"e_1_2_12_17_1","DOI":"10.1111\/nmo.14674"},{"doi-asserted-by":"publisher","key":"e_1_2_12_18_1","DOI":"10.3389\/fncel.2015.00430"},{"doi-asserted-by":"publisher","key":"e_1_2_12_19_1","DOI":"10.1113\/jphysiol.2009.177105"},{"doi-asserted-by":"publisher","key":"e_1_2_12_20_1","DOI":"10.1038\/s41598-020-70216-6"},{"doi-asserted-by":"publisher","key":"e_1_2_12_21_1","DOI":"10.1152\/ajpgi.1993.265.5.G1005"},{"doi-asserted-by":"publisher","key":"e_1_2_12_22_1","DOI":"10.1111\/j.1469-7793.2001.00741.x"},{"doi-asserted-by":"publisher","key":"e_1_2_12_23_1","DOI":"10.1007\/BF01148318"},{"doi-asserted-by":"publisher","key":"e_1_2_12_24_1","DOI":"10.1152\/ajpgi.1993.265.1.G156"},{"doi-asserted-by":"publisher","key":"e_1_2_12_25_1","DOI":"10.1016\/j.neuroscience.2017.12.052"},{"doi-asserted-by":"publisher","key":"e_1_2_12_26_1","DOI":"10.1113\/JP284171"},{"doi-asserted-by":"publisher","key":"e_1_2_12_27_1","DOI":"10.1007\/s004410051075"},{"doi-asserted-by":"publisher","key":"e_1_2_12_28_1","DOI":"10.1152\/ajpgi.00053.2003"},{"doi-asserted-by":"publisher","key":"e_1_2_12_29_1","DOI":"10.1016\/j.jchemneu.2007.03.001"},{"doi-asserted-by":"publisher","key":"e_1_2_12_30_1","DOI":"10.1113\/jphysiol.1985.sp015867"},{"doi-asserted-by":"publisher","key":"e_1_2_12_31_1","DOI":"10.1152\/ajpgi.1992.263.6.G838"},{"doi-asserted-by":"publisher","key":"e_1_2_12_32_1","DOI":"10.1152\/ajpgi.1994.266.2.G230"},{"doi-asserted-by":"publisher","key":"e_1_2_12_33_1","DOI":"10.1007\/bf00171343"}],"container-title":["Neurogastroenterology &amp; 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