{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T07:27:45Z","timestamp":1769758065076,"version":"3.49.0"},"reference-count":61,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The traditional herbal medicine <jats:italic>Atractylodes macrocephala<\/jats:italic> Koidz. (<jats:italic>A.\u2009macrocephala<\/jats:italic>) is commonly utilized for alleviating symptoms associated with spleen deficiency, abdominal distension, diarrhea, and constipation. These pharmacological effects are attributed to a variety of active constituents. However, the specific bioactive compounds responsible for promoting defecation and gastrointestinal transit in A.\u2009macrocephala remain unidentified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The primary polysaccharide characteristics of PAMK was elucidated by HPLC, FT\u2010IR, and HGPGC. Efficacy of PAMK (0.07, 0.14, and 0.28\u2009mg\/g) on mice was evaluated in a spleen deficiency constipation mouse model by analyzing stool parameters, constipation\u2010related physiological indexes, and SCFAs. The expression levels of 5\u2010HT<jats:sub>3<\/jats:sub>R, 5\u2010HT<jats:sub>4<\/jats:sub>R, and related receptor genes were examined by RT\u2010qPCR, and neurotransmitters were examined using ELISA. Finally, the diversity of gut microbiota was analyzed with 16S rDNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The results showed that PAMK significantly reduced the gastrointestinal transport time and increased the number of fecal pellets and fecal water content in spleen deficiency constipation model mice. PAMK kept the balance of 5\u2010HT, SCFAs, TPH\u20101, SERT, CgA, and neurotransmitter levels (VIP, SP, MTL) in mice colon. In addition, PAMK could regulate the abundance of gut microbiota such as <jats:italic>Alistopes<\/jats:italic>, <jats:italic>Bacteroides<\/jats:italic>, and <jats:italic>Odoribacter<\/jats:italic> in spleen deficiency constipation model mice gut.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>It can be concluded that PAMK effectively ameliorated the symptoms of spleen deficiency constipation in mice by modulating the expression of 5\u2010HT and its associated receptors. The underlying mechanism was elucidated, providing a solid theoretical foundation for the therapeutic application of <jats:italic>A.\u2009macrocephala<\/jats:italic> in treating spleen deficiency constipation and offering potential for developing novel approaches to address this condition.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14875","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T09:23:01Z","timestamp":1722331381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Polysaccharide extracted from <i>Atractylodes macrocephala<\/i> improves the spleen deficiency constipation in mice by regulating the gut microbiota to affect the 5\u2010<scp>HT<\/scp> synthesis"],"prefix":"10.1111","volume":"36","author":[{"given":"Lei","family":"Chen","sequence":"first","affiliation":[{"name":"College of Food and Health Zhejiang Agriculture and Forestry University  Hangzhou 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