{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:42:00Z","timestamp":1790480520558,"version":"4.1.0"},"reference-count":76,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,6]],"date-time":"2024-10-06T00:00:00Z","timestamp":1728172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic constipation is a gastrointestinal functional disorder which affects patient quality of life. Therefore, many studies were oriented to search herbal laxative agents. In this study, we investigated the phytochemical composition of beetroot juice (BJ) and its laxative potential in an experimental model of constipation and colonic dysmotility induced by loperamide (LOP) in <jats:italic>Wistar<\/jats:italic> rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Animals were concurrently pretreated with LOP (3\u2009mg\/kg, <jats:italic>b<\/jats:italic>.<jats:italic>w<\/jats:italic>., <jats:italic>i<\/jats:italic>.<jats:italic>p<\/jats:italic>.) and BJ (5 and 10\u2009mL\/kg, <jats:italic>b.w.<\/jats:italic>, <jats:italic>p<\/jats:italic>.<jats:italic>o<\/jats:italic>.), or yohimbine (2\u2009mg\/kg, <jats:italic>b<\/jats:italic>.<jats:italic>w.<\/jats:italic>, <jats:italic>i.p<\/jats:italic>.), during 1\u2009week. The laxative activity was determined based on the weight, frequency, and water content of the feces matter. The gastric\u2010emptying test and intestinal transit were determined. Colon histology was examined, and oxidative status was evaluated using biochemical\u2010colorimetric methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key results<\/jats:title><jats:p>The in\u00a0vivo study revealed that LOP induced a significant inhibition of gastrointestinal motility, negative consequences on defecation parameters, oxidative stress, and colonic mucosa lesions. Conversely, administration of BJ reestablished these parameters and restored colonic oxidative balance. Importantly, BJ treatment protected against LOP\u2010induced inflammatory markers (pro\u2010inflammatory cytokines and WBC) and the increase in intracellular mediators such as hydrogen peroxide, free iron, and calcium levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; inferences<\/jats:title><jats:p>This study demonstrated that the bioactive compounds in BJ provided an anti\u2010constipation effect by modulating intestinal motility and regulating oxidative stress and inflammation induced by LOP intoxication.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14935","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T04:40:32Z","timestamp":1728276032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Laxative and purgative actions of phytoactive compounds from beetroot juice against loperamide\u2010induced constipation, oxidative stress, and inflammation in rats"],"prefix":"10.1111","volume":"37","author":[{"given":"Ala","family":"Ayari","sequence":"first","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources University of Jendouba, Higher Institute of Biotechnology of B\u00e9ja  B\u00e9ja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouha","family":"Dakhli","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources University of Jendouba, Higher Institute of Biotechnology of B\u00e9ja  B\u00e9ja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saber","family":"Jedidi","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources University of Jendouba, Higher Institute of Biotechnology of B\u00e9ja  B\u00e9ja Tunisia"},{"name":"Sylvo\u2010Pastoral Institute of Tabarka, Laboratory of Sylvo\u2010Pastoral Resources University of Jendouba  Tabarka Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houcem","family":"Sammari","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources University of Jendouba, Higher Institute of Biotechnology of B\u00e9ja  B\u00e9ja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Arrari","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources University of Jendouba, Higher Institute of Biotechnology of B\u00e9ja  B\u00e9ja Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0131-1401","authenticated-orcid":false,"given":"Hichem","family":"Sebai","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources University of Jendouba, Higher Institute of Biotechnology of 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