{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T05:44:24Z","timestamp":1783748664092,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T00:00:00Z","timestamp":1736294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Postoperative ileus (POI) is an iatrogenic disorder marked by temporary impaired gastrointestinal (GI) motility post\u2010abdominal surgery. Surgical handling of the intestine activates resident macrophages (Mfs), leading to inflammatory cytokine release and leukocyte recruitment into the <jats:italic>muscularis<\/jats:italic>, which compromises intestinal contractility. The mechanisms behind this activation are unclear. Recent studies suggest peritoneal Mfs, particularly large peritoneal macrophages (LPMs), might play a role in sterile intestinal inflammation by rapidly recruiting to the serosal layer of the gut and aiding in tissue damage resolution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To identify immune cells involved in the early phase of POI, single\u2010cell RNA sequencing (scRNA\u2010seq) was conducted. The migration of LPMs post\u2010surgery was studied using adoptive transfer techniques. LPMs were depleted via intraperitoneal injection of clodronate liposomes. Subsequently, flow cytometry, quantitative PCR (qPCR), and immunofluorescence were performed to assess the impact of LPM depletion and analyze cell populations and inflammatory effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>(1) Intestinal manipulation (IM) leads to the accumulation of monocytes, neutrophils, mature Mfs, CD8+ T cells, and LPMs within 2\u2009h post\u2010surgery. (2) Heparin treatment does not affect gut transit or reduce IL\u20106, IL\u20101a, and IL\u20101b expression in the early phase of POI. (3) Depletion of LPMs via clodronate liposome does not prevent monocyte, neutrophil, and Mfs infiltration in the <jats:italic>muscularis externa<\/jats:italic>, nor does it improve gut transit or reduce cytokine expression. (4) LPMs migrate to the serosa after IM but do not enter the <jats:italic>muscularis externa<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Inferences<\/jats:title><jats:p>LPMs adhere to the intestinal serosa following intestinal manipulation but do not migrate into the intestinal <jats:italic>muscularis<\/jats:italic> or participate in the inflammatory response and delayed transit. Consequently, LPMs are not involved in the pathogenesis of POI.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.14997","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T11:06:09Z","timestamp":1736334369000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Large Peritoneal Macrophages Play No Role in the Pathogenesis of Postoperative Ileus Induced by Intestinal Manipulation"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5913-3990","authenticated-orcid":false,"given":"Zheng","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Intestinal Neuro\u2010Immune Interactions, Translational Research Center for GI Disorders (TARGID), Department of Chronic Diseases, Metabolism and Ageing KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Modave","sequence":"additional","affiliation":[{"name":"Center for Intestinal Neuro\u2010Immune Interactions, Translational Research Center for GI Disorders (TARGID), Department of Chronic Diseases, Metabolism and Ageing KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Delfini","sequence":"additional","affiliation":[{"name":"Center for Intestinal Neuro\u2010Immune Interactions, Translational Research Center for GI Disorders (TARGID), Department of Chronic Diseases, Metabolism and Ageing KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Appeltans","sequence":"additional","affiliation":[{"name":"Center for Intestinal Neuro\u2010Immune Interactions, Translational Research Center for GI Disorders (TARGID), Department of Chronic Diseases, Metabolism and Ageing KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Boeckxstaens","sequence":"additional","affiliation":[{"name":"Center for Intestinal Neuro\u2010Immune Interactions, Translational Research Center for GI Disorders (TARGID), Department of Chronic Diseases, Metabolism and Ageing KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Stakenborg","sequence":"additional","affiliation":[{"name":"Center for Intestinal Neuro\u2010Immune Interactions, Translational Research Center for GI Disorders (TARGID), Department of Chronic Diseases, Metabolism and Ageing KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/ijgc-2019-000633"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2016_108"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgs.12307"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/bjsopen\/zrad054"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2015.07.007"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1999.0029900378"},{"issue":"4","key":"e_1_2_11_8_1","first-page":"1137","article-title":"Postoperative Ileus Is Maintained by Intestinal Immune Infiltrates That Activate Inhibitory Neural Pathways in Mice","volume":"125","author":"Jonge W. 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