{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:42:18Z","timestamp":1782837738803,"version":"3.54.5"},"reference-count":85,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study emphasizes evaluating the antiemetic efficacy of the natural food component caffeic acid (CAF) using a copper sulfate pentahydrate (CuSO<jats:sub>4<\/jats:sub>.5H<jats:sub>2<\/jats:sub>O)\u2010induced emetic model on chicks, and an in silico approach was also adopted to estimate the possible underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two doses (25 and 50\u2009mg\/kg b.w.) of CAF and several referral drugs considered positive controls (PCs), including domperidone (6\u2009mg\/kg), hyoscine (21\u2009mg\/kg), aprepitant (16\u2009mg\/kg), diphenhydramine (10\u2009mg\/kg), and ondansetron (5\u2009mg\/kg), were orally administered to chicks. The vehicle served as the control group. Co\u2010treatments of CAF with referral drugs were also provided to chicks to evaluate the modulatory action of the test compound.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>According to the results, CAF delayed the emetic onset and decreased the frequency of retches in a dose\u2010dependent manner compared to the vehicle group. CAF (40\u2009mg\/kg) represented a notable delayed latency period (60.17\u2009\u00b1\u20093.16\u2009s) and a diminished number of retchings (18.33\u2009\u00b1\u20091.74 times) compared to the control group. Further, in the co\u2010treatments, CAF increased the latency period and reduced the number of retches, except for domperidone. In the in silico investigation, CAF showed notable binding affinity toward the D<jats:sub>2<\/jats:sub> (\u22127.3\u2009kcal\/mol), 5HT<jats:sub>3<\/jats:sub> (\u22127.1\u2009kcal\/mol), and M<jats:sub>5<\/jats:sub> (\u22127\u2009kcal\/mol) receptors in the same binding site as the referral ligand.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This research indicated that CAF has mild antiemetic properties by interacting with the D<jats:sub>2<\/jats:sub>, 5TH<jats:sub>3<\/jats:sub>, and M<jats:sub>5<\/jats:sub> receptors. Therefore, several preclinical and clinical studies are necessary to assess the effectiveness and safety profile of this food ingredient.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.70003","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T05:54:31Z","timestamp":1739858071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring the Antiemetic Potential of Caffeic Acid: A Combined In\u00a0Vivo and Computational Approach"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6179-9720","authenticated-orcid":false,"given":"Md. Shimul","family":"Bhuia","sequence":"first","affiliation":[{"name":"Department of Pharmacy Bangabandhu Sheikh Mujibur Rahman Science and Technology University  Dhaka Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  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