{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:35:54Z","timestamp":1792089354468,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic constipation is a prevalent, burdensome gastrointestinal disorder whose etiology and pathophysiology remain poorly understood. Differences in the composition of the intestinal microbiota have been shown between constipated patients and healthy people. Data indicate that these microbial differences contribute to the disorder.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Preclinical studies in mice examined the effects of <jats:styled-content style=\"fixed-case\"><jats:italic>Lactobacillus gasseri<\/jats:italic><\/jats:styled-content> on intestinal motility ex\u00a0vivo, the reversal of motility inhibition by \u03bc\u2010opioid receptor agonists ex\u00a0vivo and in\u00a0vivo in mice, and the effects on capsaicin\u2010stimulated transient receptor potential vanilloid 1 (TRPV1) in Jurkat cells. Thereafter, a clinical study of 40 women with functional constipation was conducted to investigate the effects of <jats:styled-content style=\"fixed-case\"><jats:italic>Lactobacillus gasseri<\/jats:italic><\/jats:styled-content> with a randomized parallel design. After 14\u2009days of baseline recording, treatment with <jats:styled-content style=\"fixed-case\"><jats:italic>Lactobacillus gasseri<\/jats:italic><\/jats:styled-content> or placebo was given over 28\u2009days, with 14\u2009days of follow\u2010up. Outcomes with complete spontaneous bowel movements (CSBM), spontaneous bowel movements, emptying frequency, abdominal pain, time spent for defecation, Bristol stool form scale, use of rescue laxatives, and impact on sex life were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>In preclinical studies, <jats:styled-content style=\"fixed-case\"><jats:italic>Lactobacillus gasseri<\/jats:italic><\/jats:styled-content> increased intestinal motility in an ex\u00a0vivo model, reversed the motility inhibition caused by \u03bc\u2010opioid receptor agonist ex\u00a0vivo and in\u00a0vivo in mice, and counteracted capsaicin\u2010stimulated activity of TRPV1 in Jurkat cells. In the clinical trial, <jats:styled-content style=\"fixed-case\"><jats:italic>Lactobacillus gasseri<\/jats:italic><\/jats:styled-content> showed a significant reduction in abdominal pain, along with a correlation and tendency for an increased number of CSBM. Few adverse events were encountered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>Treatment with <jats:styled-content style=\"fixed-case\"><jats:italic>Lactobacillus gasseri<\/jats:italic><\/jats:styled-content> can alleviate pain sensations in functional constipation, possibly with an improved bowel\u2010emptying function.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.70012","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T20:54:50Z","timestamp":1741726490000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Therapeutic Value of <i>Lactobacillus gasseri<\/i> 345A in Chronic Constipation"],"prefix":"10.1111","volume":"37","author":[{"given":"Stefan","family":"Roos","sequence":"first","affiliation":[{"name":"Department of Molecular Sciences, Uppsala BioCenter Swedish University of Agricultural Sciences  Uppsala Sweden"},{"name":"BioGaia AB  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atti\u2010La","family":"Dahlgren","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Kang","family":"Mao","sequence":"additional","affiliation":[{"name":"Brain\u2010Body Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Pallin","sequence":"additional","affiliation":[{"name":"Department of Molecular Sciences, Uppsala BioCenter Swedish University of Agricultural Sciences  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Stanisz","sequence":"additional","affiliation":[{"name":"Brain\u2010Body Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Forsythe","sequence":"additional","affiliation":[{"name":"Brain\u2010Body Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Kunze","sequence":"additional","affiliation":[{"name":"Brain\u2010Body Institute McMaster University  Hamilton Ontario Canada"},{"name":"Department of Psychiatry McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8428-0772","authenticated-orcid":false,"given":"Per M.","family":"Hellstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2007.07.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.14335"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-022-07709-z"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.8.2.107"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.071746"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12145"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2005.06.008"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/107602960100700416"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00082.2014"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11467.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20020433"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.2000.01349.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01364.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1958.sp005940"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.12306"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000023"},{"issue":"3","key":"e_1_2_10_18_1","first-page":"237","article-title":"Rome III: New Standard for Functional Gastrointestinal Disorders","volume":"15","author":"Drossman D. 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