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This study investigates the protective effects of fresh red beet juice (<jats:styled-content style=\"fixed-case\">FBRJ<\/jats:styled-content>) on gastrointestinal complications caused by co\u2010administration of dextran sulfate sodium (<jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content>) and loperamide (<jats:styled-content style=\"fixed-case\">LOP<\/jats:styled-content>), which induce ulcerative colitis and constipation, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult rats were divided into groups and subjected to a 7\u2010day treatment with 5% <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content> to induce ulcerative colitis, followed by <jats:styled-content style=\"fixed-case\">LOP<\/jats:styled-content> (3\u2009mg\/kg, body weight [b.w.]) for 7\u2009days to cause constipation. <jats:styled-content style=\"fixed-case\">FBRJ<\/jats:styled-content> (5 and 10\u2009mL\/kg, b.w.) or yohimbine (<jats:styled-content style=\"fixed-case\">YOH<\/jats:styled-content>) (2\u2009mg\/kg, b.w.) was administered 1 h after <jats:styled-content style=\"fixed-case\">LOP<\/jats:styled-content> each day for 7\u2009days. Therapeutic outcomes were evaluated based on macroscopic and histological changes in the gastrointestinal tract, gastric emptying, gastrointestinal transit, oxidative stress parameters, and inflammatory markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>FBRJ significantly alleviated gastrointestinal dysfunctions caused by DSS and\/or LOP, improving both gastric emptying and gastrointestinal transit in a dose\u2010dependent manner (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Specifically, compared with the ulcerative\/constipated group, the animals treated with the FBRJ showed a significant increase (52.43%\u2009\u00b1\u20094.65% to 66.23%\u2009\u00b1\u20096.78%) of gastric emptying (GE) andgastrointestinal transit (GIT: 48.08%\u2009\u00b1\u20093.32% to 62.46%\u2009\u00b1\u20094.98%) in a dose\u2010dependent manner. It also modulated antioxidant defense systems by inducing enzyme activities and reducing lipid peroxidation, which had been significantly disrupted by the combined effects of DSS and LOP. Furthermore, inflammatory markers, including C\u2010reactive protein (CRP), pro\u2010inflammatory cytokines, and white blood cell counts, were significantly reduced in both plasma and colonic mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Interferences<\/jats:title><jats:p>We suggest that <jats:styled-content style=\"fixed-case\">FBRJ<\/jats:styled-content> significantly protects against <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content>\u2010induced colitis and <jats:styled-content style=\"fixed-case\">LOP<\/jats:styled-content>\u2010induced constipation, involving several mechanisms such as increasing secretion and peristaltic activity, reducing inflammation, and preserving the antioxidant properties.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.70064","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T14:41:55Z","timestamp":1746196915000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Fresh Beetroot Juice Alleviates Combined Ulcerative Colitis and Constipation by Restoring Physiological and Biochemical Balances in a Murine Model"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5295-5480","authenticated-orcid":false,"given":"Ala","family":"Ayari","sequence":"first","affiliation":[{"name":"Laboratory of Functional Physiology and Valorization of Bio\u2010Resources (LR23ES08) Higher Institute of Biotechnology of B\u00e9ja, University of Jendouba  Beja 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