{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T21:08:48Z","timestamp":1758056928567,"version":"3.44.0"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["23ym0126805j0002"],"award-info":[{"award-number":["23ym0126805j0002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Peritoneal dialysis (PD) is a renal replacement therapy approach to treat end\u2010stage renal failure. However, complications such as gastrointestinal dysmotility occur in patients undergoing PD, and the mechanisms underlying these complications have not been elucidated. We hypothesized that inflammation and dysfunction of the interstitial cells of Cajal (ICC) contribute to the PD\u2010induced gastrointestinal dysmotility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were intraperitoneally administered a dialysate containing methylglyoxal (40\u2009mM) every other day for 2\u2009weeks to mimic the gastrointestinal complications in patients undergoing long\u2010term PD. The gastrointestinal transit capacity was evaluated using fluorescent dyes that were forcibly administered orally. To evaluate the inflammation and function of the ICC in the intestinal muscles, we performed real\u2010time polymerase chain reaction and immunohistochemical staining and measured spontaneous contractions ex\u00a0vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The intestinal transit capacity was significantly reduced in the methylglyoxal\u2010treated group compared to that in the control group. In the inflammatory evaluation, the number of neutrophils and macrophages in the intestinal muscles significantly increased in the methylglyoxal\u2010treated group compared to the control group. Moreover, the mRNA expression levels of <jats:italic>Tnf<\/jats:italic>, <jats:italic>Il1b<\/jats:italic>, and <jats:italic>Il6<\/jats:italic> were upregulated in the intestinal muscle from the methylglyoxal\u2010treated group. The mRNA expression of <jats:italic>Kit<\/jats:italic>, an interstitial cell of Cajal marker, was significantly decreased in the methylglyoxal\u2010treated group. In addition, the frequency of spontaneous contractions, an index of ICC function, was decreased in the methylglyoxal\u2010treated group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inference<\/jats:title><jats:p>Our data suggest that the PD\u2010induced gastrointestinal dysmotility might be due to inflammation and dysfunction of the ICC in intestinal muscles.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.70068","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T14:40:14Z","timestamp":1746196814000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intestinal Dysmotility and Associated Disorders in Intestinal Muscle of Methylglyoxal\u2010Treated Mice"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3788-0517","authenticated-orcid":false,"given":"Yuki","family":"Yamakawa","sequence":"first","affiliation":[{"name":"Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1611-8329","authenticated-orcid":false,"given":"Taiki","family":"Mihara","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Hori","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61601-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2022020221"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-9987.13450"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2020.05.032"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.34067\/KID.0000672019"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000494145"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/089686080902902S15"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfl745"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/089686080502500618"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.22-0014"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1540\/jsmr.59.1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/089686080602600317"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2014.116"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-017-0576-7"},{"key":"e_1_2_10_16_1","article-title":"Assessment of Gastric Emptying in Non\u2010Obese Diabetic Mice Using a [13C]\u2010Octanoic Acid Breath Test","volume":"73","author":"Creedon C. 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