{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:13:55Z","timestamp":1790187235376,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,31]],"date-time":"2025-08-31T00:00:00Z","timestamp":1756598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013058","name":"Jiangsu Provincial Key Research and Development Program","doi-asserted-by":"publisher","award":["BE2022673"],"award-info":[{"award-number":["BE2022673"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20231132"],"award-info":[{"award-number":["BK20231132"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Visceral hypersensitivity (VH) is a key pathophysiological feature of irritable bowel syndrome (IBS), contributing to chronic abdominal pain and discomfort. While electroacupuncture (EA) has demonstrated efficacy in alleviating IBS symptoms, the mechanisms underlying its effects at the Baliao acupoint remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this translational study, we enrolled 40 IBS patients (gender\u2010balanced, aged 30\u201360\u2009years) who received standardized EA treatment at Baliao acupoints, with pain intensity assessed using visual analogue scale (VAS) scoring. An IBS rat model was established using acetic acid enema combined with restraint stress. Rats received EA at Baliao or sham acupuncture for 2\u2009weeks. Visceral sensitivity was assessed via abdominal withdrawal reflex (AWR), while mast cell (MC) numbers, histamine and substance P (SP) release, and tight junction proteins (ZO\u20101, occludin) were quantified. Gut\u2010microbiota composition was analyzed by 16S rDNA sequencing, and the alterations of TRPV1<jats:sup>+<\/jats:sup> neuron and MAPK signaling were evaluated via immunofluorescence and Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EA at Baliao significantly attenuated VH in both IBS patients (reduced VAS scores, difference \u22123.000\u2009\u00b1\u20090.4264) and rats (lower AWR scores, difference 1.050\u2009\u00b1\u20090.2630), compared to the sham group. Treatment with EA restored gut microbial diversity, promoting beneficial taxa (Bifidobacterium, Akkermansia) while suppressing pathobionts (Helicobacter, Prevotella). Moreover, EA inhibited MC degranulation, reducing histamine (\u219340%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) and SP (\u219328%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and enhanced intestinal barrier integrity via ZO\u20101\/occludin upregulation. Crucially, EA suppressed TRPV1+ neuron activation in the colon and dorsal root ganglia, disrupting MC\u2010neuron crosstalk through TRPV1\/MAPK pathway modulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>EA (Baliao) alleviates VH by modulating gut microbiota, inhibiting MC activation, and blocking TRPV1 positive neuron mediated signal transmission. These findings highlight its potential as a non\u2010pharmacological therapy for IBS, targeting multiple pathological axes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.70137","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T05:26:51Z","timestamp":1756704411000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Electroacupuncture at Baliao Points Attenuates Visceral Hypersensitivity in Irritable Bowel Syndrome via Gut\u2010Microbiota\u2010Mast Cell\u2010<scp>TPRV1<\/scp> Axis Modulation"],"prefix":"10.1111","volume":"37","author":[{"given":"Meng\u2010Yang","family":"Sun","sequence":"first","affiliation":[{"name":"Nanjing Hospital of Chinese Medicine Nanjing University of Chinese Medicine  Nanjing China"},{"name":"Lianyungang Clinical Medical School of Nanjing Medical University  Lianyungang 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