{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T03:28:45Z","timestamp":1769743725287,"version":"3.49.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Colorectal cancer (CRC) is a leading cause of cancer\u2010related deaths, significantly disrupting enteric neurotransmission within the colon. While the effects of CRC on the enteric nervous system (ENS) of the colon are well documented, its impact on the small intestine remains underexplored. This study aims to investigate the influence of colorectal carcinogenesis on the small intestine's ENS and evaluate the individual neuroprotective effects of microencapsulated quercetin and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Bifidobacterium animalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Wistar rats were subjected to chemically induced colorectal carcinogenesis, followed by 14\u2009weeks of treatment with microencapsulated quercetin and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>B. animalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Gastrointestinal transit times were assessed, and colonic and jejunal samples underwent histopathological and immunohistochemical analyses to evaluate neuronal markers (HuC\/D, nNOS, VIP). Cholinergic neurons were not directly assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Aberrant crypt foci confirmed the effectiveness of the colorectal carcinogenesis induction model. The mean gastric emptying time (MGET) was notably shorter in the\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>B. animalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      \u2010treated group. Colorectal carcinogenesis significantly reduced the density and size of HuC\/D+ neurons in the myenteric and submucosal plexuses of the jejunum. Treatments with either microencapsulated quercetin or\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>B. animalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      significantly enhanced neuronal density and size in the jejunum and improved nitrergic neuronal density (nNOS\u2010IR). Additionally, VIPergic neuron density increased in the submucosal plexus, and varicosity size increased in the myenteric plexus in the CR group; treatments reduced this varicosity size.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study provides the first evidence that colorectal carcinogenesis damages jejunal neurons. Treatments with microencapsulated quercetin or\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>B. animalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      independently preserved neuronal density and modulated gastrointestinal function. However, their combined administration did not enhance these effects, highlighting the need for further research into therapeutic interventions for preserving ENS integrity during colorectal carcinogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70190","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T04:25:56Z","timestamp":1761798356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microencapsulated Quercetin and\n                    <i>Bifidobacterium animalis<\/i>\n                    Independently Preserve Jejunal Enteric Neurons During Colorectal Carcinogenesis"],"prefix":"10.1111","volume":"38","author":[{"given":"Lucas","family":"Casagrande","sequence":"first","affiliation":[{"name":"Pharmaceutical Sciences Graduate Program University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla Cristina de Oliveira","family":"Bernardo","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Graduate Program University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina Silva","family":"Sestak","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Graduate Program University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maysa Pacheco Alvarez","family":"da Silva","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Graduate Program University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Yudi Nagaoka","family":"Godoy","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Graduate Program University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2506-6252","authenticated-orcid":false,"given":"Jean\u2010Pierre","family":"Timmermans","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology &amp; Histology University of Antwerp  Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar Agostinho","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Health Sciences Graduate Program State University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia Cristina Alexandrino","family":"Becker","sequence":"additional","affiliation":[{"name":"Health Sciences Graduate Program State University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erick Guilherme","family":"Stoppa","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waldiceu Aparecido","family":"Verri","sequence":"additional","affiliation":[{"name":"State University of Londrina  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Ricardo de Arruma","family":"Miranda","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6701-2917","authenticated-orcid":false,"given":"Juliana Vanessa Colombo","family":"Martins Perles","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Graduate Program University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2892-5123","authenticated-orcid":false,"given":"Jacqueline Nelisis","family":"Zanoni","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Graduate Program University of Maring\u00e1  Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i12.2642"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18061310"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21103494"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/CANCERS13010152"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.32"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26595"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CBI.2010.03.048"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2020.102759"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/2050312119825784"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/IJMS22094906"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.8.5.1073"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1208\/S12249\u2010012\u20109752\u20100\/TABLES\/4"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.EJPB.2006.04.003"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12660"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/NMO.13824"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1088\/0967\u20103334\/31\/2\/003"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1118\/1.596832"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1590\/S2175\u201097902024E23717"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.FCT.2009.12.003"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304\u20103835(87)90157\u20101"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100264.2005.00584.x"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0162998"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2982.2002.00317.x"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.120985"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010004\u20100426\u2010x"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.15640\/ijhs.v3n1a10"},{"issue":"1","key":"e_1_2_11_29_1","first-page":"61","article-title":"The Dimethylhydrazine Induced Colorectal Tumours in Rat\u2014Experimental Colorectal Carcinogenesis","volume":"39","author":"Perse M.","year":"2005","journal-title":"Radiation Oncology"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/TOXRES\/TFAA004"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.864"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2017.08.003"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/IJMS21093391"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1365\u20102982.2007.01023.X"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/BIOM13111586"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.18388\/ABP.2015_1154"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362\u2010028X.JFP\u201017\u2010214"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.12688\/F1000RESEARCH.18039.1"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470988756"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1179\/147683010X12611460763841"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10042\u2010010\u20100029\u20108"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.AMSU.2015.07.018"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/FIMMU.2013.00226\/BIBTEX"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.NEULET.2006.05.058"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41598\u2010023\u201028073\u20106"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/S13277\u2010014\u20101852\u2010X\/FIGURES\/4"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/02841860214957"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737\u2010199711000\u201000002"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1476\u20105381.1992.TB13412.X"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2016_103"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.4249\/scholarpedia.4064"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.940500"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.2337\/DB11\u20101019"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415\u201052732010000400013"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ABB.2015.08.008"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.TOXREP.2014.07.014"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2020.01.011"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BIOPHA.2017.05.008"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119910"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00150\u201017"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/FOODS12020320"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010024\u201000626\u20101"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.70190","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nmo.70190","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.70190","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:14:26Z","timestamp":1769660066000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.70190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/nmo.70190"],"URL":"https:\/\/doi.org\/10.1111\/nmo.70190","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]},"assertion":[{"value":"2024-08-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70190"}}