{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:16:26Z","timestamp":1789470986720,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      This study investigated the effects of moxibustion on ferroptosis and oxidative stress in the colon tissue of Crohn's disease (\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      ) mice by regulating Piezo1 and explored its potential mechanism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      was induced using 1.5% dextran sodium sulfate (\n                      <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content>\n                      ) in drinking water. After the moxibustion intervention, the disease activity index (\n                      <jats:styled-content style=\"fixed-case\">DAI<\/jats:styled-content>\n                      ) score and intestinal propulsion rate were assessed. Histopathological changes in colon tissue were examined using hematoxylin\u2013eosin staining, and mitochondrial morphology in colon epithelial cells was observed via transmission electron microscopy. Immunofluorescence was used to detect\n                      <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\n                      , Piezo1,\n                      <jats:styled-content style=\"fixed-case\">GPX4<\/jats:styled-content>\n                      , and\n                      <jats:styled-content style=\"fixed-case\">FTH<\/jats:styled-content>\n                      expression in colon tissue. Enzyme\u2010linked immunosorbent assay was performed to measure inflammatory cytokine levels, while oxidative stress\u2010 and ferroptosis\u2010related protein expression was analyzed by immunoblotting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content>\n                      \u2010induced model group exhibited excessive intestinal motility, severe colonic tissue damage, elevated inflammatory cytokines (\n                      <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\n                      \u2010\u03b1,\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u20101\u03b2, and\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201017), upregulated Piezo1 expression, mitochondrial swelling, reduced mitochondrial cristae, decreased expression of ferroptosis\u2010related proteins (\n                      <jats:styled-content style=\"fixed-case\">SLC7A11<\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">GPX4<\/jats:styled-content>\n                      , and\n                      <jats:styled-content style=\"fixed-case\">FTH1<\/jats:styled-content>\n                      ), and increased\n                      <jats:styled-content style=\"fixed-case\">ACSL4<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">LPCAT3<\/jats:styled-content>\n                      expression. Moxibustion significantly reduced the\n                      <jats:styled-content style=\"fixed-case\">DAI<\/jats:styled-content>\n                      score and intestinal propulsion rate, alleviated colonic tissue damage, lowered inflammatory cytokine levels, downregulated Piezo1 expression, restored mitochondrial morphology, reduced oxidative stress, and inhibited ferroptosis\u2010related protein dysregulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Moxibustion alleviated oxidative stress and ferroptosis in the colon tissue of\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      mice, thereby reducing intestinal inflammation. Its mechanism may involve the regulation of Piezo1, a mechanosensitive ion channel.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70219","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T17:05:59Z","timestamp":1764349559000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Moxibustion Alleviates Intestinal Inflammation by Down\u2010Regulating Piezo1 to Inhibit Ferroptosis in Colonic Tissues of Crohn's Disease Mice"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8302-1983","authenticated-orcid":false,"given":"Jingying","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion, Tuina and Rehabilitation Hunan University of Chinese Medicine  Changsha 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