{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T09:42:36Z","timestamp":1781948556096,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Recent work suggests an altered duodenal mucosa\u2010associated microbiota (d\u2010MAM) in patients with functional dyspepsia (FD) when compared to controls. This may reflect alterations in host\u2010microbiome homeostasis. Given the specific mucosal immune signatures identified in FD, we hypothesize that these signatures are associated with specific microbial changes. We aim to profile the d\u2010MAM to identify microbes associated with known changes in FD mucosal and peripheral immunity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Upper gastrointestinal biopsies were collected from 11 outpatient controls and 17 FD patients. Specific biopsies were collected for 16S rRNA sequencing, histology, and mucosal lamina propria mononuclear cell (LPMC) isolation. Where available, peripheral blood mononuclear cells (PBMC) were isolated. PBMC and LPMC populations were analyzed for T\u2010cell populations by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      Comparing the histological and immune measures between FD and controls revealed significant differences with decreased villi goblet cells and increased LPMC CD4 Central Memory, LPMC CD8, and PBMC CD4+ Central Memory Th17 in FD patients. Specific microbiome associations found that in controls, villi goblet cells positively correlated with\n                      <jats:italic>Massilia<\/jats:italic>\n                      and negatively with\n                      <jats:italic>Exiguobacterium<\/jats:italic>\n                      . Additionally, controls had a negative correlation between LPMC CD4 Central Memory and\n                      <jats:italic>Veillonella<\/jats:italic>\n                      . Notably, FD patients demonstrated a significant negative correlation between LPMC CD8 and\n                      <jats:italic>Sulfophobococcus<\/jats:italic>\n                      , and a positive correlation between PBMC CD4+ Central Memory Th17 and both\n                      <jats:italic>Gemella<\/jats:italic>\n                      and\n                      <jats:italic>Fusobacterium<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Inferences<\/jats:title>\n                    <jats:p>Our findings contribute to a growing body of evidence, indicating FD patients exhibit distinct alterations in d\u2010MAM and immune profiles compared to controls. Furthermore, the immune\u2010microbiome associations within control populations were absent in FD patients, suggesting a loss of host\u2010microbiome homeostasis that may contribute to FD pathophysiology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70238","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T14:34:32Z","timestamp":1769610872000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Altered Duodenal Mucosa\u2010Associated Microbiota and Immune Profiles in Functional Dyspepsia: A Study of Host\u2010Microbiome Homeostasis"],"prefix":"10.1111","volume":"38","author":[{"given":"Emily C.","family":"Hoedt","sequence":"first","affiliation":[{"name":"College of Health, Medicine and Wellbeing University of Newcastle  Callaghan New South Wales Australia"},{"name":"Hunter Medical Research Institute Immune Health Research Program  New Lambton Heights New South Wales Australia"},{"name":"Centre of Research Excellence in Transforming Gut Health, College of Health, Medicine and Wellbeing University of Newcastle  Newcastle New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace L.","family":"Burns","sequence":"additional","affiliation":[{"name":"College of Health, Medicine and Wellbeing University of Newcastle  Callaghan New South Wales Australia"},{"name":"Hunter Medical Research Institute Immune Health Research Program  New Lambton Heights New South Wales Australia"},{"name":"Centre of Research Excellence in Transforming Gut Health, College of Health, Medicine and Wellbeing University of Newcastle  Newcastle New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seungha","family":"Kang","sequence":"additional","affiliation":[{"name":"Frazer Institute, Faculty of Health, Medicine and Behavioural Sciences The University of Queensland  Brisbane Australia"},{"name":"Centre of Research Excellence in Transforming Gut Health University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Bruce","sequence":"additional","affiliation":[{"name":"College of Health, Medicine and Wellbeing University of Newcastle  Callaghan New South Wales Australia"},{"name":"Hunter Medical Research Institute Immune Health Research Program  New Lambton Heights New South Wales Australia"},{"name":"Centre of Research Excellence in Transforming Gut Health, College of Health, Medicine and Wellbeing University of Newcastle  Newcastle New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Morrison","sequence":"additional","affiliation":[{"name":"Frazer Institute, Faculty of Health, Medicine and Behavioural Sciences The University of Queensland  Brisbane Australia"},{"name":"Centre of Research Excellence in Transforming Gut Health University of Queensland  Brisbane Australia"},{"name":"Department of Gastroenterology and Hepatology Princess Alexandra Hospital  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1248-9590","authenticated-orcid":false,"given":"Simon","family":"Keely","sequence":"additional","affiliation":[{"name":"College of Health, Medicine and Wellbeing University of Newcastle  Callaghan New South Wales Australia"},{"name":"Hunter Medical Research Institute Immune Health Research Program  New Lambton Heights New South Wales Australia"},{"name":"Centre of Research Excellence in Transforming Gut Health, College of Health, Medicine and Wellbeing University of Newcastle  Newcastle New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2537-3092","authenticated-orcid":false,"given":"Nicholas J.","family":"Talley","sequence":"additional","affiliation":[{"name":"College of Health, Medicine and Wellbeing University of Newcastle  Callaghan New South Wales Australia"},{"name":"Hunter Medical Research Institute Immune Health Research Program  New Lambton Heights New South Wales Australia"},{"name":"Centre of Research Excellence in Transforming Gut Health, College of Health, Medicine and Wellbeing University of Newcastle  Newcastle New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-54716-3"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.02.011"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.1051632","article-title":"Type 2 and Type 17 Effector Cells Are Increased in the Duodenal Mucosa but Not Peripheral Blood of Patients With Functional Dyspepsia","volume":"13","author":"Burns G. 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