{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T14:07:13Z","timestamp":1780063633233,"version":"3.54.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["Grant# ANR\u201023\u2010CE16\u20100018"],"award-info":[{"award-number":["Grant# ANR\u201023\u2010CE16\u20100018"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004431","name":"Fondation de France","doi-asserted-by":"publisher","award":["Grant#00160116\/WB\u20102024\u201055195"],"award-info":[{"award-number":["Grant#00160116\/WB\u20102024\u201055195"]}],"id":[{"id":"10.13039\/501100004431","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Enteroendocrine cells (EECs) are dispersed along the intestinal mucosa and transduce luminal stimuli into hormonal signals. EECs exhibit neuron\u2010like features and express both \u03b1\u2010synuclein and tau, two proteins pathologically and genetically linked to Parkinson disease (PD). These observations support the hypothesis that EECs may be involved in disease development in the \u201cbody\u2010first\u201d PD subtype. Cellular models represent invaluable tools for studying the role of \u03b1\u2010synuclein and tau in PD pathogenesis. However, the sensitivity and specificity of commercial antibodies for detecting \u03b1\u2010synuclein and tau in EEC cell lines remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We tested by immunoblot a panel of commercial total\u2010\u03b1\u2010synuclein and total\u2010tau antibodies on protein lysates from three EEC cell lines: GLUTag, NCI\u2010H716, and STC\u20101. Pharmacological and biochemical manipulations were applied to assess the specificity of antibodies against phosphorylated \u03b1\u2010synuclein and tau.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>Five antibodies detected total \u03b1\u2010synuclein in NCI\u2010H716 lysates, whereas the antibodies D1M9X and Tau12 detected total tau in GLUTag and NCI\u2010H716 lysates, respectively. \u03b1\u2010synuclein and tau protein levels were comparable between na\u00efve and differentiated NCI\u2010H716 cells. Four phospho\u2010specific antibodies revealed phospho\u2010\u03b1\u2010synuclein S129 in NCI\u2010H716. Of the nine phospho\u2010tau antibodies tested, six recognized tau phosphorylated at specific epitopes (T181, S199, T231, S356, S396, and S404) in GLUTag cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Inferences<\/jats:title>\n                    <jats:p>Our results indicate that NCI\u2010H716 cells represent an ideal EEC model for studying \u03b1\u2010synuclein expression and phosphorylation, whereas GLUTag cells are preferable for investigating tau protein biology. This work provides a comprehensive antibody toolbox to dissect the physiological and pathological role of \u03b1\u2010synuclein and tau in EECs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70259","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T04:30:21Z","timestamp":1770265821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of \u03b1\u2010Synuclein and Tau Detection by Immunoblot in Enteroendocrine Cell Lines"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9979-8961","authenticated-orcid":false,"given":"Giuseppe","family":"Madaro","sequence":"first","affiliation":[{"name":"INSERM, CHU Nantes, the Enteric Nervous System in Gut and Brain Disorders Nantes Universit\u00e9  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2033-4846","authenticated-orcid":false,"given":"Pierre\u2010Fran\u00e7ois","family":"Charbonneau","sequence":"additional","affiliation":[{"name":"INSERM, CHU Nantes, the Enteric Nervous System in Gut and Brain Disorders Nantes Universit\u00e9  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibauld","family":"Oullier","sequence":"additional","affiliation":[{"name":"INSERM, CHU Nantes, the Enteric Nervous System in Gut and Brain Disorders Nantes Universit\u00e9  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Neunlist","sequence":"additional","affiliation":[{"name":"INSERM, CHU Nantes, the Enteric Nervous System in Gut and Brain Disorders Nantes Universit\u00e9  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwenola","family":"Le Dr\u00e9an","sequence":"additional","affiliation":[{"name":"INRAE, IMAD, CRNH\u2010O, UMR1280, PhAN Nantes Universit\u00e9  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8792-2582","authenticated-orcid":false,"given":"Pascal","family":"Derkinderen","sequence":"additional","affiliation":[{"name":"INSERM, CHU Nantes, the Enteric Nervous System in Gut and Brain Disorders Nantes Universit\u00e9  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2018.11.014"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-019-0168-8"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.92295"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2023.1166848"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-15-0293"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-08076-5"},{"issue":"8","key":"e_1_2_11_8_1","article-title":"The Poly\u2010Arginine Peptide R18D Interferes With the Internalisation of \u03b1\u2010Synuclein Pre\u2010Formed Fibrils in STC\u20101 Enteroendocrine Cells","volume":"11","author":"Gorecki A. 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