{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:13:44Z","timestamp":1790187224976,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Irritable bowel syndrome (IBS) increases the risk of depression and damages the body, but there is no ideal treatment. However, aberrant expression of miRNAs in gastrointestinal diseases holds promise for the treatment of IBS. The aim of this study was to investigate how miR\u2010149\u20105p regulates IBS by affecting the function of colonic mucosal epithelial cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We examined miR\u2010149\u20105p and IGF2BP1 levels in colonic tissue from clinical patients. The mice model of IBS was established by acetic acid (AA) enema, and NCM460 was induced using LPS in order to establish a cellular model. Measurement of fecal weight and water content in mice evaluated the model. miR\u2010149\u20105p, IGF2BP1, IL\u20101\u03b2, and IL\u20106 levels were detected by qRT\u2010PCR. The targeting relationship between miR\u2010149\u20105p and IGF2BP1 was detected by dual\u2010luciferase reporter gene assay, and cell proliferation was examined by CCK\u20108. Elisa was used to detect D\u2010LA concentration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      miR\u2010149\u20105p levels were up\u2010regulated in the colon of patients with IBS, whereas the opposite was true for IGF2BP1 levels. AA enemas exacerbated the degree of diarrhea in mice, but diarrhea and inflammation were less severe in miR\u2010149\u20105p\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice. miR\u2010149\u20105p negatively regulates IGF2BP1. IGF2BP1 partially reversed the enhanced proliferation and inflammatory suppression of NCM460 cells caused by silencing miR\u2010149\u20105p.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Inferences<\/jats:title>\n                    <jats:p>Inhibition of miR\u2010149\u20105p protects colonic mucosal epithelial cells and alleviates IBS by negatively regulating IGF2BP1.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70331","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T06:25:46Z","timestamp":1778826346000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Down\u2010Regulation of\n                    <scp>miR<\/scp>\n                    \u2010149\u20105p Regulates Colon Mucosal Epithelial Cell Function by Targeting\n                    <scp>IGF2BP1<\/scp>\n                    to Alleviate Irritable Bowel Syndrome"],"prefix":"10.1111","volume":"38","author":[{"given":"Xiaotong","family":"He","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Shanghai Pudong New Area People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Shanghai Pudong New Area People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingmei","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Shanghai Pudong New Area People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieru","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Shanghai Pudong New Area People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.25122\/jml-2022-0089"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26010413"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-0286-8"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.22532"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524022666220811161111"},{"issue":"9","key":"e_1_2_12_7_1","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1016\/j.dld.2024.01.209","article-title":"The Role of miRNA in IBS Pathogenesis, Diagnosis and Therapy: The Latest Thought","volume":"56","author":"Zhang X.","year":"2024","journal-title":"Digestive and Liver Disease: Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the 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