{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:58:08Z","timestamp":1785473888276,"version":"3.56.0"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Transient receptor potential melastatin 8 (TRPM8) is a cold\u2010sensitive Ca\n                      <jats:sup>2+<\/jats:sup>\n                      \u2010permeable ion channel that regulates intestinal inflammation. This study was aimed to elucidate the role of TRPM8 in enteric macrophages and spinal afferent sensory neurons during dextran sulfate sodium (DSS)\u2013induced colitis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The role of TRPM8 in the progression of colonic inflammation was examined in 2% DSS\u2010induced colitis by immunohistochemistry, reverse transcription\u2010polymerase chain reaction, visceral sensing assessment, and flow cytometry. The role of TRPM8 in sensory neurons and macrophages was investigated using primary cultured dorsal root ganglion (DRG) neurons and bone marrow\u2010derived macrophages (BMDMs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>DSS\u2010induced colitis was significantly aggravated in TRPM8\u2010deficient (KO) mice compared with that in wild\u2010type (WT) mice. In the colitis model, KO mice showed lower IL\u201010 mRNA levels than WT mice, but IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1 levels were not affected. TRPM8 expression was upregulated in the distal colon and DRG following DSS administration. In the distal colon, TRPM8\u2010immunoreactive cells and nerve fibers colocalized with F4\/80 and substance P\/calcitonin gene\u2010related peptide (CGRP), respectively. In the DRG, TRPM8 neuron subtypes were labeled by neurofilament 200, substance P, CGRP, and tyrosine kinase receptor A. In cultured DRG neurons, TRPM8 deficiency attenuated lipopolysaccharide (LPS)\u2013elicited CGRP release but did not affect substance P release. In BMDMs, TRPM8 deficiency significantly decreased LPS\u2010 and LPS\/CGRP\u2010elicited upregulation of IL\u201010 mRNA compared with that in WT animals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions &amp; Inferences<\/jats:title>\n                    <jats:p>TRPM8 protects against DSS\u2010induced experimental colitis via regulating CGRP release from sensory neurons and IL\u201010 production in enteric macrophages during intestinal inflammation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70399","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:20:27Z","timestamp":1784262027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transient Receptor Potential Melastatin 8 Plays a Key Role in Dextran Sulfate Sodium\u2010Induced Murine Colitis via Regulating Extrinsic Sensory Neurons and Enteric Macrophage Secretory Activities"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5690-5958","authenticated-orcid":false,"given":"Kenjiro","family":"Matsumoto","sequence":"first","affiliation":[{"name":"Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics Kyoto Pharmaceutical University  Kyoto Japan"},{"name":"Laboratory of Pathophysiology, Faculty of Pharmaceutical Sciences Doshisha Women's College of Liberal Arts  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Nakamoto","sequence":"additional","affiliation":[{"name":"Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics Kyoto Pharmaceutical University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akari","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Pathological Sciences, Department of Pharmacology and 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