{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:01:15Z","timestamp":1790661675296,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Aging increases the risk of bowel dysfunction and reduced quality of life. Although Neurokinin A (NKA)\u2010mediated smooth muscle contraction declines with age, it is unclear whether this reflects altered NKA content and\/or release. This study examined age\u2010related changes in colonic NKA content and release and assessed whether changes correlated with fecal NKA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>An electrochemical immunosensor was developed and characterized for NKA detection. Distal colon tissue and fecal pellets were collected from 5\u2010 and 22\u2010month\u2010old female C57BL6J mice. Basal and mechanically evoked tissue NKA release was recorded in the presence and absence of ondansetron. NKA content in tissue and fecal pellets were measured.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Sensor fabrication was validated by electrochemical and EDX analyses. The immunosensor detected NKA with a detection limit of 2.76\u2009pM and high selectivity. Basal and mechanically evoked NKA release were significantly reduced with age. Ondansetron inhibited mechanically stimulated NKA release in 5\u2010month\u2010old but not 22\u2010month\u2010old colons. Tissue NKA content declined with age. The proportion of total NKA released decreased from 65%\u2009\u00b1\u200922% in young tissue to 30%\u2009\u00b1\u200923% in aged tissue. Fecal NKA levels were similarly reduced in older mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Reduced NKA release with aging is associated with lower tissue content. The electrochemical immunosensor provides a simple, cost\u2010effective method for NKA quantification and could be adapted for other gastrointestinal neuropeptides. The decrease in fecal NKA levels supports further investigation of fecal NKA as a noninvasive marker of age\u2010related changes in bowel function.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70446","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:38:26Z","timestamp":1790231906000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Age\u2010Related Decreases in Colonic Neurokinin A Content and Release Monitored Using Electrochemical Immunosensors"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8572-8735","authenticated-orcid":false,"given":"Chloe","family":"Miller","sequence":"first","affiliation":[{"name":"School of Applied Sciences University of Brighton  Brighton UK"},{"name":"Centre for Lifelong Health University of Brighton  Brighton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2113-8650","authenticated-orcid":false,"given":"Mark","family":"Yeoman","sequence":"additional","affiliation":[{"name":"School of Applied Sciences University of Brighton  Brighton UK"},{"name":"Centre for Lifelong Health University of Brighton  Brighton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-3850","authenticated-orcid":false,"given":"Bhavik Anil","family":"Patel","sequence":"additional","affiliation":[{"name":"School of Applied Sciences University of Brighton  Brighton UK"},{"name":"Centre for Lifelong Health University of Brighton  Brighton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afac147"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2017.03.008"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-008045046-9.01474-1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-21-06975.1996"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(96)00195-7"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4892(01)00100-X"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2014.02.007"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13426"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.14891"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2006.02.014"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2025.171419"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2019.305359"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2019.09.001"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v8.i1.172"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12791"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/VIW.20200102"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2024.117949"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.11.029"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios9030086"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biosx.2025.100667"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2024.135694"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2025.146401"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2020.136618"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-27656-7"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/celc.202200831"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134157"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2376-3_10"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.3c06200"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2018.03.004"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP271403"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42754"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1752-153X-6-31"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/cn4000617"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2.2.110"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpainsymman.2008.04.016"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.04114.x"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12937"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00144.2019"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.70446","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nmo.70446","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.70446","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:22:58Z","timestamp":1790659378000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.70446"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/nmo.70446"],"URL":"https:\/\/doi.org\/10.1111\/nmo.70446","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70446"}}