{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T07:40:48Z","timestamp":1792395648397,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["833816\u2010NEUMACS"],"award-info":[{"award-number":["833816\u2010NEUMACS"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["11C2219N"],"award-info":[{"award-number":["11C2219N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Obese patients suffer from cardiovascular, metabolic and gastrointestinal disorders, including constipation, proposed to result from high fat diet (HFD)\u2010induced enteric neuron loss. However, these studies used high\u2010fat purified diets, containing reduced amounts of fiber compared to non\u2010purified diets, known to alter gut physiology and microbiome composition. Therefore, we evaluated the effect of purified HFD\u2010induced obesity on enteric neuron density and gastrointestinal transit compared to its proper control diet, that is, a purified control diet.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Wild type mice were fed a standard chow diet, purified low fat diet (LFD) or HFD. Colonic transit was measured via glass bead expulsion following 12, 18, and 24\u2009weeks of diet. Colonic muscularis externa was collected for immunofluorescent stainings of enteric neurons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Starting from 18\u2009weeks of HFD, a decline in enteric neuron number was observed compared to LFD. In addition, delayed colonic transit was observed in HFD\u2010fed mice when compared to LFD\u2010fed animals after 18\u2009weeks of exposure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study demonstrates that prolonged exposure to a HFD results in a reduced enteric neuron density and delayed colonic transit, and underscores the need to use a proper control diet when evaluating the impact of a HFD on gut physiology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nmo.70470","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T06:54:18Z","timestamp":1792392858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High Fat Diet Alters Gastrointestinal Function and Results in Reduced Enteric Neuron Density"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1116-8397","authenticated-orcid":false,"given":"Marta","family":"Chavero\u2010Pieres","sequence":"first","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0660-9506","authenticated-orcid":false,"given":"Maria Francesca","family":"Viola","sequence":"additional","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"},{"name":"Developmental Biology of the Immune System, Life &amp; Medical Sciences (LIMES) Institute University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5913-3990","authenticated-orcid":false,"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0342-5445","authenticated-orcid":false,"given":"Marcello","family":"Delfini","sequence":"additional","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Appeltans","sequence":"additional","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9316-3869","authenticated-orcid":false,"given":"Naomi","family":"Fabre","sequence":"additional","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8267-5797","authenticated-orcid":false,"given":"Guy","family":"Boeckxstaens","sequence":"additional","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6229-0045","authenticated-orcid":false,"given":"Nathalie","family":"Stakenborg","sequence":"additional","affiliation":[{"name":"Laboratory for Intestinal Neuro\u2010Immune Interaction, Translational Research Center for Gastrointestinal Disorders, Department of Chronic Diseases Metabolism and Ageing, KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,18]]},"reference":[{"issue":"5","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1038\/s41574-019-0176-8","article-title":"Obesity: Global Epidemiology and Pathogenesis","volume":"15","author":"Bluher M.","year":"2019","journal-title":"Nature Reviews. 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