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Resistance against the fungal pathogen\n                          <jats:italic>Leptosphaeria maculans<\/jats:italic>\n                          in\n                          <jats:italic>Arabidopsis<\/jats:italic>\n                          requires abscisic acid (\n                          <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\n                          ), which promotes the deposition of callose, a \u03b2\u20101,3\u2010glucan polymer. Here, we examined the role of\n                          <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                          2 in callose deposition in relation to\n                          <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\n                          treatment and challenge with\n                          <jats:italic>L.\u00a0maculans<\/jats:italic>\n                          and\n                          <jats:italic>Pseudomonas syringae<\/jats:italic>\n                          .\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Characterization of\n                          <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                          2\u2010overexpressing plants and the knockout line indicated that\n                          <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                          2 negatively affects callose deposition. Recombinant\n                          <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                          2 purified from\n                          <jats:italic>Pichia pastoris<\/jats:italic>\n                          showed callose\u2010degrading activity, and a considerable reduction in the callose\u2010degrading activity was observed in the leaf extract of the\n                          <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                          2 knockout line compared with the wild\u2010type.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\n                          pretreatment before challenge with\n                          <jats:italic>L.\u00a0maculans<\/jats:italic>\n                          concomitantly repressed\n                          <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                          2 and enhanced callose accumulation. Likewise, overexpression of an\n                          <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\n                          biosynthesis gene\n                          <jats:styled-content style=\"fixed-case\">NCED<\/jats:styled-content>\n                          3 resulted in reduced\n                          <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                          2 expression and increased callose deposition.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          We propose that\n                          <jats:styled-content style=\"fixed-case\">ABA<\/jats:styled-content>\n                          promotes callose deposition through the transcriptional repression of\n                          <jats:italic>\n                            <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                            2\n                          <\/jats:italic>\n                          in\n                          <jats:italic>Arabidopsis<\/jats:italic>\n                          challenged by\n                          <jats:italic>L.\u00a0maculans<\/jats:italic>\n                          and\n                          <jats:italic>P.\u00a0syringae<\/jats:italic>\n                          . Callose by itself is likely to act antagonistically on salicylic acid (\n                          <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\n                          ) defense signaling, suggesting that\n                          <jats:italic>\n                            <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>\n                            2\n                          <\/jats:italic>\n                          may function as a modulator of callose\u2010 and\n                          <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\n                          \u2010dependent defense responses.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.12436","type":"journal-article","created":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T05:22:54Z","timestamp":1376889774000},"page":"1187-1199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":151,"title":["A novel role of\n                    <scp>PR<\/scp>\n                    2 in abscisic acid (\n                    <scp>ABA<\/scp>\n                    ) mediated, pathogen\u2010induced callose deposition in\n                    <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1111","volume":"200","author":[{"given":"Shinichi","family":"Oide","sequence":"first","affiliation":[{"name":"Department of Plant Biology and Forest Genetics Uppsala BioCenter Swedish University of Agricultural Sciences and Linnean Center for Plant Biology PO Box 7080 750 07 Uppsala 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