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However, there has been no report that a viral capsid protein may traffic within a cell by utilizing the actin\/endoplasmic reticulum (<jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>) network.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We used <jats:italic>Tomato spotted wilt tospovirus<\/jats:italic> (<jats:styled-content style=\"fixed-case\">TSWV<\/jats:styled-content>) as a model virus to study the cell biological properties of a nucleocapsid (N) protein.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We found that <jats:styled-content style=\"fixed-case\">TSWV<\/jats:styled-content> N protein was capable of forming highly motile cytoplasmic inclusions that moved along the <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> and actin network. The disruption of actin filaments by latrunculin B, an actin\u2010depolymerizing agent, almost stopped the intracellular movement of N inclusions, whereas treatment with a microtubule\u2010depolymerizing reagent, oryzalin, did not. The over\u2010expression of a myosin <jats:styled-content style=\"fixed-case\">XI<\/jats:styled-content>\u2010K tail, functioning in a dominant\u2010negative manner, completely halted the movement of N inclusions. Latrunculin B treatment strongly inhibited the formation of <jats:styled-content style=\"fixed-case\">TSWV<\/jats:styled-content> local lesions in <jats:italic>Nicotiana tabacum<\/jats:italic> cv Samsun <jats:styled-content style=\"fixed-case\">NN<\/jats:styled-content> and delayed systemic infection in <jats:italic>N.\u00a0benthamiana<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Collectively, our findings provide the first evidence that the capsid protein of a plant virus has the novel property of intracellular trafficking. The findings add capsid protein as a new class of viral protein that traffics on the actin\/<jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> system.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.12447","type":"journal-article","created":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T08:18:53Z","timestamp":1377245933000},"page":"1212-1224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Nucleocapsid of <i>Tomato spotted wilt tospovirus<\/i> forms mobile particles that traffic on an actin\/endoplasmic reticulum network driven by myosin <scp>XI<\/scp>\u2010K"],"prefix":"10.1111","volume":"200","author":[{"given":"Zhike","family":"Feng","sequence":"first","affiliation":[{"name":"Key Laboratory of Integrated Management of Crop Diseases and Pests Ministry of Education Department of Plant Pathology Nanjing Agricultural University Nanjing 210095 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