{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:05:08Z","timestamp":1790805908650,"version":"4.1.0"},"reference-count":73,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T00:00:00Z","timestamp":1390262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":588,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>The gaseous plant hormone ethylene is involved in many physiological processes including climacteric fruit ripening, in which it is a key determinant of fruit quality. A detailed model that describes ethylene biochemistry dynamics is missing. Often, kinetic modeling is used to describe metabolic networks or signaling cascades, mostly ignoring the link with transcriptomic data.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We have constructed an elegant kinetic model that describes the transfer of genetic information into abundance and metabolic activity of proteins for the entire ethylene biosynthesis pathway during fruit development and ripening of tomato (<jats:italic>Solanum lycopersicum<\/jats:italic>).<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our model was calibrated against a vast amount of transcriptomic, proteomic and metabolic data and showed good descriptive qualities. Subsequently it was validated successfully against several ripening mutants previously described in the literature. The model was used as a predictive tool to evaluate novel and existing hypotheses regarding the regulation of ethylene biosynthesis.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>This bottom\u2010up kinetic network model was used to indicate that a side\u2010branch of the ethylene pathway, the formation of the dead\u2010end product 1\u2010(malonylamino)\u20101\u2010aminocyclopropane\u20101\u2010carboxylic acid (<jats:styled-content style=\"fixed-case\">MACC<\/jats:styled-content>), might have a strong effect on eventual ethylene production. Furthermore, our <jats:italic>in silico<\/jats:italic> analyses indicated potential (post\u2010) translational regulation of the ethylene\u2010forming enzyme <jats:styled-content style=\"fixed-case\">ACC<\/jats:styled-content> oxidase.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.12685","type":"journal-article","created":{"date-parts":[[2014,1,21]],"date-time":"2014-01-21T12:28:28Z","timestamp":1390307308000},"page":"952-963","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["A transcriptomics\u2010based kinetic model for ethylene biosynthesis in tomato (<i>Solanum lycopersicum<\/i>) fruit: development, validation and exploration of novel regulatory mechanisms"],"prefix":"10.1111","volume":"202","author":[{"given":"Bram","family":"Van de Poel","sequence":"first","affiliation":[{"name":"Division of MeBioS Department of Biosystems (BIOSYST) KU Leuven Willem de Croylaan 42, bus 2428 3001 Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Bulens","sequence":"additional","affiliation":[{"name":"Division of MeBioS Department of Biosystems (BIOSYST) KU Leuven Willem de Croylaan 42, bus 2428 3001 Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten L. 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