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However, the contribution of this pathway in regulating virulence of plant pathogenic fungi is unknown.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>We identified and characterized nine genes encoding components of the<jats:styled-content style=\"fixed-case\">TOR<\/jats:styled-content>pathway in<jats:italic>Fusarium graminearum<\/jats:italic>. Biological, genetic and biochemical functions of each component were investigated.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>The FgFkbp12\u2010rapamycin complex binds to the FgTor kinase. The type 2A phosphatases FgPp2A, FgSit4 and FgPpg1 were found to interact with FgTap42, a downstream component of FgTor. Among these, we determined that FgPp2A is likely to be essential for<jats:italic>F.\u00a0graminearum<\/jats:italic>survival, and FgSit4 and FgPpg1 play important roles in cell wall integrity by positively regulating the phosphorylation of FgMgv1, a key<jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>kinase in the cell wall integrity pathway. In addition, the FgPpg1 interacting protein, FgTip41, is involved in regulating mycelial growth and virulence. Notably, FgTip41 does not interact with FgTap42 but with FgPpg1, suggesting the existence of FgTap42:FgPpg1:FgTip41 heterotrimer in<jats:italic>F.\u00a0graminearum<\/jats:italic>, a complex not observed in the yeast model.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Collectively, we defined a genetic regulatory framework that elucidates how the<jats:styled-content style=\"fixed-case\">TOR<\/jats:styled-content>pathway regulates virulence and vegetative development in<jats:italic>F.\u00a0graminearum<\/jats:italic>.<\/jats:p><\/jats:list-item><\/jats:list><\/jats:p>","DOI":"10.1111\/nph.12776","type":"journal-article","created":{"date-parts":[[2014,3,28]],"date-time":"2014-03-28T23:39:25Z","timestamp":1396049965000},"page":"219-232","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":162,"title":["The<scp>TOR<\/scp>signaling pathway regulates vegetative development and virulence in<i>Fusarium graminearum<\/i>"],"prefix":"10.1111","volume":"203","author":[{"given":"Fangwei","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Biotechnology Zhejiang University Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Gu","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Zhejiang University Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzi","family":"Yun","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Zhejiang University Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanni","family":"Yin","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Zhejiang University Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Rong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Pathology Purdue University West Lafayette IN 47907\u20102054 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won\u2010Bo","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology Texas A&amp;M University College Station TX 77843\u20102132 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghua","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology Zhejiang University Hangzhou 310058 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