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An Arabidopsis thaliana<\/jats:italic> double mutant in two type III<\/jats:styled-content> phosphatidylinositol\u20104\u2010kinases (PI<\/jats:styled-content>4Ks), pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic>, displays a stunted rosette growth. The causal link between PI<\/jats:styled-content>4K activity and growth is unknown.<\/jats:p><\/jats:list-item>\n\nUsing microarray analysis, quantitative reverse transcription polymerase chain reaction (RT\u2010qPCR<\/jats:styled-content>) and multiple phytohormone analysis by LC\u2010MS we investigated the mechanism responsible for the pi4kIII\u03b21\u03b22<\/jats:italic> phenotype.<\/jats:p><\/jats:list-item>\n\nThe pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> mutant accumulated a high concentration of salicylic acid (SA<\/jats:styled-content>), constitutively expressed SA<\/jats:styled-content> marker genes including PR<\/jats:styled-content>\u20101<\/jats:italic>, and was more resistant to Pseudomonas syringae<\/jats:italic>. pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> was crossed with SA<\/jats:styled-content> signalling mutants eds1<\/jats:italic> and npr1<\/jats:italic> and SA<\/jats:styled-content> biosynthesis mutant sid2<\/jats:italic> and NahG<\/jats:italic>. The dwarf phenotype of pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> rosettes was suppressed in all four triple mutants. Whereas eds1 pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic>, sid2 pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> and NahG pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> had similar amounts of SA<\/jats:styled-content> as the wild\u2010type (WT<\/jats:styled-content>), npr1pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> had more SA<\/jats:styled-content> than pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> despite being less dwarfed. This indicates that PI<\/jats:styled-content>4KIII<\/jats:styled-content>\u03b21<\/jats:italic> and PI<\/jats:styled-content>4KIII<\/jats:styled-content>\u03b22<\/jats:italic> are genetically upstream of EDS<\/jats:styled-content>1<\/jats:italic> and need functional SA<\/jats:styled-content> biosynthesis and perception through NPR<\/jats:styled-content>1 to express the dwarf phenotype. The slow root growth phenotype of pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> was not suppressed in any of the triple mutants.<\/jats:p><\/jats:list-item>\n\nThe pi4kIII<\/jats:styled-content>\u03b21\u03b22<\/jats:italic> mutations together cause constitutive activation of SA<\/jats:styled-content> signalling that is responsible for the dwarf rosette phenotype but not for the short root phenotype.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.12822","type":"journal-article","created":{"date-parts":[[2014,4,24]],"date-time":"2014-04-24T08:25:49Z","timestamp":1398327949000},"page":"805-816","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Constitutive salicylic acid accumulation in pi4kIII<\/scp>\u03b21\u03b22<\/i> Arabidopsis plants stunts rosette but not root growth"],"prefix":"10.1111","volume":"203","author":[{"given":"Vladim\u00edr","family":"\u0160a\u0161ek","sequence":"first","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of Czech Republic Prague 165 02 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Janda","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of Czech Republic Prague 165 02 Czech Republic"},{"name":"Department of Biochemistry and Microbiology Institute of Chemical Technology Prague 166 28 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Delage","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris 6 UR5 Physiologie Cellulaire et Mol\u00e9culaire des Plantes 75252 Paris Cedex 05 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette","family":"Puyaubert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris 6 UR5 Physiologie Cellulaire et Mol\u00e9culaire des Plantes 75252 Paris Cedex 05 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Guivarc'h","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris 6 UR5 Physiologie Cellulaire et Mol\u00e9culaire des Plantes 75252 Paris Cedex 05 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Encarnaci\u00f3n","family":"L\u00f3pez Maseda","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of Czech Republic Prague 165 02 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petre I.","family":"Dobrev","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of Czech Republic Prague 165 02 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Caius","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en G\u00e9nomique V\u00e9g\u00e9tale (URGV) Plateforme Transcriptome UMR INRA 1165 Universit\u00e9 d'Evry Val d'Essonne \u2013 ERL CNRS 8196 2 rue G. 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