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The localization of these potential self\u2010toxicants has not been proven.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We investigated the localization of these compounds in leaves using <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>aman microscopy. The results are presented as heat maps derived from principal component analysis (<jats:styled-content style=\"fixed-case\">PCA<\/jats:styled-content>) of the <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>aman spectra from sampling grids of leaf sections. This approach used undirected, data\u2010driven analysis to qualitatively distinguish localized plant chemistry. The presence of \u03b2\u2010triketones and lipophilic flavonoids was confirmed by <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> and <jats:sup>1<\/jats:sup><jats:styled-content style=\"fixed-case\">H NMR<\/jats:styled-content> spectroscopy.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Grandiflorone was compartmentalized within the leaf oil glands of <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>.\u00a0morrisonii<\/jats:italic>. <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>eptospermum scoparium<\/jats:italic> also contained high concentrations of grandiflorone, previously reported as only a trace component in essential oils, localized in the oil glands in the leaves of varieties from diverse geographical locations. Raman microscopy was used to probe the chemistry of oil glands in several ornamental m\u0101nuka varieties, revealing high concentrations of bioactive flavonoids localized in these glands.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>The compartmentalization of \u03b2\u2010triketones within oil glands inside leaves of <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>eptospermum<\/jats:italic> shrubs may defend the plants against herbicidal activity.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.12970","type":"journal-article","created":{"date-parts":[[2014,8,7]],"date-time":"2014-08-07T15:33:39Z","timestamp":1407425619000},"page":"339-349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Herbicidal \u03b2\u2010triketones are compartmentalized in leaves of <i><scp>L<\/scp>eptospermum<\/i> species: localization by <scp>R<\/scp>aman microscopy and rapid screening"],"prefix":"10.1111","volume":"205","author":[{"given":"Daniel P.","family":"Killeen","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Otago PO Box 56 Dunedin New Zealand"},{"name":"MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"van Klink","sequence":"additional","affiliation":[{"name":"Department of Chemistry The New Zealand Institute for Plant &amp; Food Research Limited University of Otago PO Box 56 Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce M.","family":"Smallfield","sequence":"additional","affiliation":[{"name":"Department of Chemistry The New Zealand Institute for Plant &amp; Food Research Limited University of Otago PO Box 56 Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith C.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Otago PO Box 56 Dunedin New Zealand"},{"name":"MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel B.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Otago PO Box 56 Dunedin New Zealand"},{"name":"Department of Chemistry The New Zealand Institute for Plant &amp; Food Research Limited University of Otago PO Box 56 Dunedin New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2009.03.020"},{"key":"e_1_2_6_3_1","volume-title":"Identification of essential oil composition by gas chromatography\/mass spectrometry","author":"Adams RP","year":"2007"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1991.tb03186.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/b500975h"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0510440"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20284"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vibspec.2006.08.004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1071\/CH9640428"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.03.015"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3NP70100J"},{"key":"e_1_2_6_12_1","volume-title":"Eucalyptus leaf oils. 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