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However, the net C<\/jats:styled-content>d2+<\/jats:sup> influx in association with H<\/jats:styled-content>+<\/jats:sup>\/C<\/jats:styled-content>a2+<\/jats:sup>, C<\/jats:styled-content>d tolerance, and the underlying molecular and physiological mechanisms are uncharacterized in these poplars.<\/jats:p><\/jats:list-item>\n\nWe assessed net C<\/jats:styled-content>d2+<\/jats:sup> influx, C<\/jats:styled-content>d tolerance and the transcriptional regulation of several genes involved in C<\/jats:styled-content>d2+<\/jats:sup> transport and detoxification in wild\u2010type and transgenic poplars.<\/jats:p><\/jats:list-item>\n\nPoplars exhibited highest net C<\/jats:styled-content>d2+<\/jats:sup> influxes into roots at pH<\/jats:styled-content> 5.5 and 0.1\u00a0mM C<\/jats:styled-content>a2+<\/jats:sup>. Transgenics had higher C<\/jats:styled-content>d2+<\/jats:sup> uptake rates and elevated transcript levels of several genes involved in C<\/jats:styled-content>d2+<\/jats:sup> transport and detoxification compared with wild\u2010type poplars. Transgenics exhibited greater C<\/jats:styled-content>d accumulation in the aerial parts than wild\u2010type plants in response to C<\/jats:styled-content>d2+<\/jats:sup> exposure. Moreover, transgenic poplars had lower concentrations of O<\/jats:styled-content>2<\/jats:sub>\u02d9\u2212<\/jats:sup> and H<\/jats:styled-content>2<\/jats:sub>O<\/jats:styled-content>2<\/jats:sub>; higher concentrations of total thiols, GSH<\/jats:styled-content> and oxidized GSH<\/jats:styled-content> in roots and\/or leaves; and stimulated foliar GSH<\/jats:styled-content> reductase activity compared with wild\u2010type plants.<\/jats:p><\/jats:list-item>\n\nThese results indicate that transgenics are more tolerant of 100\u00a0\u03bcM C<\/jats:styled-content>d2+<\/jats:sup> than wild\u2010type plants, probably due to the GSH<\/jats:styled-content>\u2010mediated induction of the transcription of genes involved in C<\/jats:styled-content>d2+<\/jats:sup> transport and detoxification.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.13013","type":"journal-article","created":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T07:57:13Z","timestamp":1410940633000},"page":"240-254","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":241,"title":["Overexpression of bacterial \u03b3\u2010glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar"],"prefix":"10.1111","volume":"205","author":[{"given":"Jiali","family":"He","sequence":"first","affiliation":[{"name":"College of Life Sciences and State Key Laboratory of Crop Stress Biology in Arid Areas Northwest A&F University Yangling Shaanxi 712100 China"},{"name":"Department of Horticulture Shenyang Agricultural University Shenyang 110866 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Key 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