{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:16:03Z","timestamp":1789622163519,"version":"build-2803163510"},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T00:00:00Z","timestamp":1414972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":302,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p>Salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>) plays a critical role in plant defense against pathogen invasion. <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010induced viral defense in plants is distinct from the pathways mediating bacterial and fungal defense and involves a specific pathway mediated by mitochondria; however, the underlying mechanisms remain largely unknown.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>The SA\u2010binding activity of the recombinant tomato (<jats:italic>Solanum lycopersicum<\/jats:italic>) alpha\u2010ketoglutarate dehydrogenase (<jats:italic>Sl<\/jats:italic>\u03b1\u2010k<jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content>) E2 subunit of the tricarboxylic acid (TCA) cycle was characterized. The biological role of this binding in plant defenses against <jats:italic>tobacco mosaic virus<\/jats:italic> (TMV) was further investigated via <jats:italic>Sl<\/jats:italic>\u03b1<jats:italic>\u2010k<jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content> E2<\/jats:italic> silencing and transient overexpression in plants.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p><jats:italic>Sl<\/jats:italic>\u03b1\u2010<jats:styled-content style=\"fixed-case\">kGDH<\/jats:styled-content> E2 was found to bind SA in two independent assays. SA treatment, as well as <jats:italic>Sl<\/jats:italic>\u03b1<jats:italic>\u2010k<jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content> E2<\/jats:italic> silencing, increased resistance to TMV. SA did not further enhance TMV defense in <jats:italic>Sl<\/jats:italic>\u03b1<jats:italic>\u2010k<jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content> E2<\/jats:italic>\u2010silenced tomato plants but did reduce TMV susceptibility in <jats:italic>Nicotiana benthamiana<\/jats:italic> plants transiently overexpressing <jats:italic>Sl<\/jats:italic>\u03b1<jats:italic>\u2010k<jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content> E2<\/jats:italic>. Furthermore, <jats:italic>Sl<\/jats:italic>\u03b1<jats:italic>\u2010k<jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content> E2<\/jats:italic>\u2010silencing\u2010induced TMV resistance was fully blocked by bongkrekic acid application and <jats:italic>alternative oxidase 1a<\/jats:italic> silencing.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>These results indicated that binding by <jats:italic>Sl<\/jats:italic>\u03b1\u2010k<jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content> E2 of SA acts upstream of and affects the mitochondrial electron transport chain, which plays an important role in basal defense against TMV. The findings of this study help to elucidate the mechanisms of SA\u2010induced viral defense.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.13137","type":"journal-article","created":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T13:46:06Z","timestamp":1415022366000},"page":"1296-1307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Salicylic acid binding of mitochondrial alpha\u2010ketoglutarate dehydrogenase E2 affects mitochondrial oxidative phosphorylation and electron transport chain components and plays a role in basal defense against <i>tobacco mosaic virus<\/i> in tomato"],"prefix":"10.1111","volume":"205","author":[{"given":"Yangwenke","family":"Liao","sequence":"first","affiliation":[{"name":"Department of Horticulture Zhejiang University Zijingang Campus 866 Yuhangtang Road Hangzhou 310058 China"},{"name":"College of Biology and the Environment Nanjing Forestry 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