{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:36:34Z","timestamp":1789382194574,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,2,10]],"date-time":"2015-02-10T00:00:00Z","timestamp":1423526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":203,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n\n<jats:list-item><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium tumefaciens<\/jats:italic> causes crown gall disease. Although <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium<\/jats:italic> can be popularly used for genetic engineering, the influence of aboveground insect infestation on <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium<\/jats:italic> induced gall formation has not been investigated.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>icotiana benthamiana<\/jats:italic> leaves were exposed to a sucking insect (whitefly) infestation and benzothiadiazole (<jats:styled-content style=\"fixed-case\">BTH<\/jats:styled-content>) for 7\u00a0d, and these exposed plants were inoculated with a tumorigenic <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium<\/jats:italic> strain. We evaluated, both <jats:italic>in planta<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>, how whitefly infestation affects crown gall disease.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Whitefly\u2010infested plants exhibited at least a two\u2010fold reduction in gall formation on both stem and crown root. Silencing of <jats:italic>isochorismate synthase 1<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">ICS<\/jats:styled-content>1<\/jats:italic>)<jats:italic>,<\/jats:italic> required for salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>) synthesis, compromised gall formation indicating an involvement of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> in whitefly\u2010derived plant defence against <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium<\/jats:italic>. Endogenous <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> content was augmented in whitefly\u2010infested plants upon <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium<\/jats:italic> inoculation. In addition, <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> concentration was three times higher in root exudates from whitefly\u2010infested plants. As a consequence, <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium\u2010<\/jats:italic>mediated transformation of roots of whitefly\u2010infested plants was clearly inhibited when compared to control plants. These results suggest that aboveground whitefly infestation elicits systemic defence responses throughout the plant.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our findings provide new insights into insect\u2010mediated leaf\u2013root intra\u2010communication and a framework to understand interactions between three organisms: whitefly, <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>.\u00a0benthamiana<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>grobacterium<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.13324","type":"journal-article","created":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T20:21:40Z","timestamp":1423772500000},"page":"148-158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Aboveground insect infestation attenuates belowground <i>Agrobacterium\u2010<\/i>mediated genetic transformation"],"prefix":"10.1111","volume":"207","author":[{"given":"Geun Cheol","family":"Song","sequence":"first","affiliation":[{"name":"Molecular Phytobacteriology Laboratory Superbacteria Research Center KRIBB Daejeon 305\u2010806 South Korea"},{"name":"Biosystems and Bioengineering Program University of Science and Technology (UST) Daejeon 305\u2010350 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soohyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Molecular Phytobacteriology Laboratory Superbacteria Research Center KRIBB Daejeon 305\u2010806 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaehwa","family":"Hong","sequence":"additional","affiliation":[{"name":"Molecular Phytobacteriology Laboratory Superbacteria Research Center KRIBB Daejeon 305\u2010806 South Korea"},{"name":"Department of Plant Pathology Chungnam National University Daejeon 305\u2010764 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