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The salicylic acid (SA<\/jats:styled-content>)\u2010inducible Oryza sativa<\/jats:italic> WRKY6 (OsWRKY6) was identified as a positive regulator of Oryza sativa pathogenesis\u2010related 10a<\/jats:italic> (OsPR10a<\/jats:italic>) by transient expression assays. A physical interaction between OsWRKY<\/jats:styled-content>6 and W\u2010box\u2010like element 1 (WLE<\/jats:styled-content>1), which positively regulates OsPR<\/jats:styled-content>10a\/probenazole induced protein<\/jats:styled-content> 1<\/jats:italic> expression, was verified in\u00a0vitro<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\nSeveral pathogenesis\u2010related (PR<\/jats:styled-content><\/jats:italic>) genes were constitutively activated, including OsPR<\/jats:styled-content>10a<\/jats:italic>, and transgenic rice (Oryza sativa<\/jats:italic>) plants overexpressing (ox) OsWRKY<\/jats:styled-content>6<\/jats:italic> exhibited enhanced disease resistance to pathogens. By contrast, PR<\/jats:styled-content><\/jats:italic> gene induction was compromised in transgenic OsWRKY<\/jats:styled-content>6<\/jats:italic>\u2010RNA<\/jats:styled-content>i lines, suggesting that OsWRKY<\/jats:styled-content>6 is a positive regulator of defense responses. OsWRKY<\/jats:styled-content>6\u2010ox lines displayed leaf lesions, and increased OsWRKY<\/jats:styled-content>6 levels caused cell death.<\/jats:p><\/jats:list-item>\n\nSalicylic acid (SA<\/jats:styled-content>) concentrations were higher in OsWRKY<\/jats:styled-content>6<\/jats:italic>\u2010ox lines than in wild\u2010type (WT<\/jats:styled-content>) plants, and transcript levels of Oryza sativa isochorismate synthase 1<\/jats:italic> (OsICS1<\/jats:italic>), which encodes a major enzyme involved in SA<\/jats:styled-content> biosynthesis, were higher in OsWRKY<\/jats:styled-content>6<\/jats:italic>\u2010ox lines than in WT<\/jats:styled-content>. OsWRKY<\/jats:styled-content>6 directly bound to the OsICS<\/jats:styled-content>1<\/jats:italic> promoter in\u00a0vivo<\/jats:italic>. This indicates that OsWRKY<\/jats:styled-content>6 can directly regulate OsICS<\/jats:styled-content>1<\/jats:italic> expression and thereby increase SA<\/jats:styled-content> concentrations. OsWRKY<\/jats:styled-content>6 autoregulates its own expression. OsWRKY<\/jats:styled-content>6 protein degradation is possibly regulated by ubiquitination.<\/jats:p><\/jats:list-item>\n\nOur results suggest that OsWRKY<\/jats:styled-content>6 positively regulates defense responses through activation of OsICS<\/jats:styled-content>1<\/jats:italic> expression and OsWRKY<\/jats:styled-content>6 stabilization.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.13516","type":"journal-article","created":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T13:37:35Z","timestamp":1434548255000},"page":"846-859","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":152,"title":["Molecular characterization of Oryza sativa <\/i>WRKY<\/scp>6, which binds to W\u2010box\u2010like element 1 of the Oryza sativa pathogenesis\u2010related<\/i> (PR<\/i>) 10a<\/i> promoter and confers reduced susceptibility to pathogens"],"prefix":"10.1111","volume":"208","author":[{"given":"Changhyun","family":"Choi","sequence":"first","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seon\u2010Hee","family":"Hwang","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Il Ran","family":"Fang","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Il","family":"Kwon","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Ryeol","family":"Park","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilpyung","family":"Ahn","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Bong","family":"Kim","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duk\u2010Ju","family":"Hwang","sequence":"additional","affiliation":[{"name":"National Academy of Agricultural Science Rural Development Administration Jeonju 560\u2010500 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