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This nutrient has to be provided by the host plant through molybdate transporters.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Members of the molybdate transporter family Molybdate Transporter type 1 (\n                          <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                          1) were identified in the model legume\n                          <jats:italic>Medicago truncatula<\/jats:italic>\n                          and their expression in nodules was determined. Yeast toxicity assays, confocal microscopy, and phenotypical characterization of a\n                          <jats:italic>Transposable Element from Nicotiana\u00a0tabacum<\/jats:italic>\n                          (\n                          <jats:italic>Tnt1<\/jats:italic>\n                          ) insertional mutant line were carried out in the one\n                          <jats:italic>M.\u00a0truncatula<\/jats:italic>\n                          <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                          1 family member specifically expressed in nodules.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Among the five\n                          <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                          1 members present in the\n                          <jats:italic>M.\u00a0truncatula<\/jats:italic>\n                          genome,\n                          <jats:italic>\n                            Mt\n                            <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                            1.3\n                          <\/jats:italic>\n                          is the only one uniquely expressed in nodules. Mt\n                          <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                          1.3 shows molybdate transport capabilities when expressed in yeast. Immunolocalization studies revealed that Mt\n                          <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                          1.3 is located in the plasma membrane of nodule cells. A\n                          <jats:italic>mot1.3\u20101<\/jats:italic>\n                          knockout mutant showed impaired growth concomitant with a reduction of nitrogenase activity. This phenotype was rescued by increasing molybdate concentrations in the nutritive solution, or upon addition of an assimilable nitrogen source. Furthermore,\n                          <jats:italic>mot1.3\u20101<\/jats:italic>\n                          plants transformed with a functional copy of\n                          <jats:italic>\n                            Mt\n                            <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                            1.3\n                          <\/jats:italic>\n                          showed a wild\u2010type\u2010like phenotype.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          These data are consistent with a model in which Mt\n                          <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>\n                          1.3 is responsible for introducing molybdate into nodule cells, which is later used to synthesize functional nitrogenase.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1111\/nph.14739","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T09:36:41Z","timestamp":1502703401000},"page":"1223-1235","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["<i>Medicago truncatula<\/i>\n                    Molybdate Transporter type 1 (MtMOT1.3) is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules under molybdenum deficiency"],"prefix":"10.1111","volume":"216","author":[{"given":"Manuel","family":"Tejada\u2010Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas (UPM\u2010INIA) Universidad Polit\u00e9cnica de Madrid Campus de Montegancedo Crta. M\u201040\u00a0km 38 28223 Pozuelo de Alarc\u00f3n (Madrid) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Gil\u2010D\u00edez","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas (UPM\u2010INIA) Universidad Polit\u00e9cnica de Madrid Campus de Montegancedo Crta. M\u201040\u00a0km 38 28223 Pozuelo de Alarc\u00f3n (Madrid) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Le\u00f3n\u2010Mediavilla","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas (UPM\u2010INIA) Universidad Polit\u00e9cnica de Madrid Campus de Montegancedo Crta. 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