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Here we analyzed the roles of transcription factors <jats:styled-content style=\"fixed-case\">TGACG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BINDING FACTOR<\/jats:styled-content> 1 (<jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>1) and <jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>4 in regulating <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> and Pip biosynthesis in <jats:italic>Arabidopsis thaliana<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We quantified the expression levels of <jats:italic>SYSTEMIC ACQUIRED RESISTANCE <jats:styled-content style=\"fixed-case\">DEFICIENT<\/jats:styled-content> 1<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1<\/jats:italic>) and <jats:italic>CALMODULIN\u2010<jats:styled-content style=\"fixed-case\">BINDING PROTEIN<\/jats:styled-content> 60g<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>60g<\/jats:italic>), which encode two master transcription factors of plant immunity, and the accumulation of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> and Pip in <jats:italic>tga1\u20101 tga4\u20101<\/jats:italic> mutant plants. We tested whether <jats:italic><jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>60g<\/jats:italic> are direct targets of <jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>1 by chromatin immunoprecipitation\u2212polymerase chain reaction (Ch<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>).<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>In addition to promoting pathogen\u2010induced <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> biosynthesis, we found that <jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>60g also positively regulated Pip biosynthesis by targeting genes encoding key biosynthesis enzymes of Pip. <jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>4 were required for full induction of <jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>60g in plant defense. Ch<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis showed that <jats:italic><jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1<\/jats:italic> was a direct target of <jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>1. In <jats:italic>tga1\u20101 tga4\u20101<\/jats:italic> mutant plants, the expression levels of <jats:italic><jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>60g<\/jats:italic> along with <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> and Pip accumulation following pathogen infection were dramatically reduced compared with those in wild\u2010type plants. Consistent with reduced expression of <jats:italic><jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>60g<\/jats:italic>, pathogen\u2010associated molecular pattern (<jats:styled-content style=\"fixed-case\">PAMP<\/jats:styled-content>)\u2010induced pathogen resistance and systemic acquired resistance were compromised in <jats:italic>tga1\u20101 tga4\u20101<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our study showed that <jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">TGA<\/jats:styled-content>4 regulate Pip and <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> biosynthesis by modulating the expression of <jats:italic><jats:styled-content style=\"fixed-case\">SARD<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">CBP<\/jats:styled-content>60g<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.14780","type":"journal-article","created":{"date-parts":[[2017,9,12]],"date-time":"2017-09-12T16:24:57Z","timestamp":1505233497000},"page":"344-354","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":184,"title":["<scp>TGACG<\/scp>\u2010<scp>BINDING FACTOR<\/scp> 1 (<scp>TGA<\/scp>1) and <scp>TGA<\/scp>4 regulate salicylic acid and pipecolic acid biosynthesis by modulating the expression of <i>SYSTEMIC ACQUIRED RESISTANCE <scp>DEFICIENT<\/scp> 1<\/i> (<i><scp>SARD<\/scp>1<\/i>) and <i><scp>CALMODULIN<\/scp>\u2010<scp>BINDING PROTEIN<\/scp> 60g<\/i> (<i><scp>CBP<\/scp>60g<\/i>)"],"prefix":"10.1111","volume":"217","author":[{"given":"Tongjun","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Botany University of British Columbia Vancouver BC V6T 1Z4 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