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However, the underlying molecular mechanisms remain to be demonstrated.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>We carried out a forward genetic screen in a root high\u2010affinity Mn transporter <jats:italic>nramp1<\/jats:italic> mutant background in <jats:italic>Arabidopsis thaliana<\/jats:italic> and identified an uncharacterized Mn transport <jats:styled-content style=\"fixed-case\">NRAMP<\/jats:styled-content>2. We investigated the effect of <jats:italic>nramp2<\/jats:italic> mutation on root growth and reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) accumulation and we also examined the <jats:italic><jats:styled-content style=\"fixed-case\">NRAMP<\/jats:styled-content>2<\/jats:italic> expression pattern, and the subcellular localization and transport activity of <jats:styled-content style=\"fixed-case\">NRAMP<\/jats:styled-content>2.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Mutation of <jats:italic>NRAMP2<\/jats:italic> impaired plant growth, while overexpression of <jats:italic>NRAMP2<\/jats:italic> improved plant growth under low Mn conditions. In the <jats:italic>nramp2\u20101nramp1<\/jats:italic> double mutant, Mn deficiency inhibited root cell elongation and root hair development, which was associated with increased hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) accumulation. <jats:styled-content style=\"fixed-case\">NRAMP<\/jats:styled-content>2 is preferentially localized to the trans\u2010Golgi network. <jats:styled-content style=\"fixed-case\">NRAMP<\/jats:styled-content>2 has Mn influx transport activity in yeast, and mutation of <jats:italic>NRAMP2<\/jats:italic> led to greater Mn retention in roots.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Our results suggest that under Mn\u2010deficient conditions, increased accumulation of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> is partially responsible for the root growth inhibition and <jats:styled-content style=\"fixed-case\">NRAMP<\/jats:styled-content>2 is involved in remobilization of Mn in Golgi for root growth.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.14783","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T07:49:44Z","timestamp":1505461784000},"page":"179-193","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":142,"title":["NRAMP2, a trans\u2010Golgi network\u2010localized manganese transporter, is required for <i>Arabidopsis<\/i> root growth under manganese deficiency"],"prefix":"10.1111","volume":"217","author":[{"given":"Huiling","family":"Gao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Genetics and Germplasm Enhancement College of Resources and Environmental Science Nanjing Agricultural University Nanjing 210095 China"},{"name":"Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai 200032 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