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This study aimed to identify structural <jats:styled-content style=\"fixed-case\">BABA<\/jats:styled-content> analogues that induce resistance without stunting plant growth.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Using site\u2010directed mutagenesis, we demonstrate that the (<jats:sc>l<\/jats:sc>)\u2010aspartic acid\u2010binding domain of <jats:styled-content style=\"fixed-case\">IBI<\/jats:styled-content>1 is critical for <jats:styled-content style=\"fixed-case\">BABA<\/jats:styled-content> perception. Based on interaction models of this domain, we screened a small library of structural <jats:styled-content style=\"fixed-case\">BABA<\/jats:styled-content> analogues for growth repression and induced resistance against biotrophic <jats:italic>Hyaloperonospora arabidopsidis<\/jats:italic> (<jats:italic>Hpa<\/jats:italic>).<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>A range of resistance\u2010inducing compounds were identified, of which (<jats:italic>R<\/jats:italic>)\u2010\u03b2\u2010homoserine (<jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content>) was the most effective. Surprisingly, <jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content> acted through different pathways than <jats:styled-content style=\"fixed-case\">BABA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content>\u2010induced resistance (<jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>) against <jats:italic>Hpa<\/jats:italic> functioned independently of salicylic acid, partially relied on camalexin, and was associated with augmented cell wall defense. <jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> against necrotrophic <jats:italic>Plectosphaerella cucumerina<\/jats:italic> acted via priming of ethylene and jasmonic acid defenses. <jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> was also effective in tomato against <jats:italic>Botrytis cinerea<\/jats:italic>. Metabolic profiling revealed that <jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content>, unlike <jats:styled-content style=\"fixed-case\">BABA<\/jats:styled-content>, does not majorly affect plant metabolism.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p><jats:styled-content style=\"fixed-case\">RBH<\/jats:styled-content> primes distinct defense pathways against biotrophic and necrotrophic pathogens without stunting plant growth, signifying strong potential for exploitation in crop protection.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.15062","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T20:36:06Z","timestamp":1519245366000},"page":"1205-1216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Chemical priming of immunity without costs to plant 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