{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:33:45Z","timestamp":1790390025099,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T00:00:00Z","timestamp":1536019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_163260"],"award-info":[{"award-number":["310030_163260"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005196","name":"Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["nph.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["New Phytologist"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Summary<\/jats:title>\n\n\nWall\u2010associated kinases (WAK<\/jats:styled-content>s) have recently been identified as major components of fungal and bacterial disease resistance in several cereal crop species. However, the molecular mechanisms of WAK<\/jats:styled-content>\u2010mediated resistance remain largely unknown.<\/jats:p><\/jats:list-item>\n\nHere, we investigated the function of the maize gene ZmWAK<\/jats:styled-content>\u2010RLK<\/jats:styled-content>1<\/jats:italic> (Htn1<\/jats:italic>) that confers quantitative resistance to northern corn leaf blight (NCLB<\/jats:styled-content>) caused by the hemibiotrophic fungal pathogen Exserohilum turcicum<\/jats:italic>.<\/jats:p><\/jats:list-item>\n\nZmWAK<\/jats:styled-content>\u2010RLK<\/jats:styled-content>1 was found to localize to the plasma membrane and its presence resulted in a modification of the infection process by reducing pathogen penetration into host tissues. A large\u2010scale transcriptome analysis of near\u2010isogenic lines (NIL<\/jats:styled-content>s) differing for ZmWAK<\/jats:styled-content>\u2010RLK<\/jats:styled-content>1<\/jats:italic> revealed that several differentially expressed genes are involved in the biosynthesis of the secondary metabolites benzoxazinoids (BX<\/jats:styled-content>s). The contents of several BX<\/jats:styled-content>s including DIM<\/jats:styled-content>2<\/jats:sub>BOA<\/jats:styled-content>\u2010Glc were significantly lower when ZmWAK<\/jats:styled-content>\u2010RLK<\/jats:styled-content>1<\/jats:italic> is present. DIM<\/jats:styled-content>2<\/jats:sub>BOA<\/jats:styled-content>\u2010Glc concentration was significantly elevated in ZmWAK<\/jats:styled-content>\u2010RLK<\/jats:styled-content>1<\/jats:italic> mutants with compromised NCLB<\/jats:styled-content> resistance. Maize mutants that were affected in overall BX<\/jats:styled-content>s biosynthesis or content of DIM<\/jats:styled-content>2<\/jats:sub>BOA<\/jats:styled-content>\u2010Glc showed increased NCLB<\/jats:styled-content> resistance.<\/jats:p><\/jats:list-item>\n\nWe conclude that Htn1<\/jats:italic>\u2010mediated NCLB<\/jats:styled-content> resistance is associated with a reduction of BX<\/jats:styled-content> secondary metabolites. These findings suggest a link between WAK<\/jats:styled-content>\u2010mediated quantitative disease resistance and changes in biochemical fluxes starting with indole\u20103\u2010glycerol phosphate.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.15419","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T08:44:39Z","timestamp":1536050679000},"page":"976-987","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":97,"title":["Fungal resistance mediated by maize wall\u2010associated kinase ZmWAK<\/scp>\u2010RLK<\/scp>1 correlates with reduced benzoxazinoid content"],"prefix":"10.1111","volume":"221","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6977-8449","authenticated-orcid":false,"given":"Ping","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology University of Zurich Zollikerstrasse 107 CH\u20108008 Zurich Switzerland"},{"name":"Institute of Crop Sciences Chinese Academy of Agricultural Sciences 100081 Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coraline","family":"Praz","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology University of Zurich Zollikerstrasse 107 CH\u20108008 Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Plant Sciences University of Bern CH\u20103013 Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti","family":"Singla","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology University of Zurich Zollikerstrasse 107 CH\u20108008 Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle A. 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